NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates.
dc.contributor.author | DeWolfe, David | |
dc.contributor.author | Aid, Malika | |
dc.contributor.author | McGann, Kevin | |
dc.contributor.author | Ghofrani, Joshua | |
dc.contributor.author | Geiger, Emma | |
dc.contributor.author | Helzer, Catherine | |
dc.contributor.author | Malik, Shaily | |
dc.contributor.author | Kleiboeker, Steve | |
dc.contributor.author | Jost, Stephanie | |
dc.contributor.author | Tan, Chen Sabrina | |
dc.date.accessioned | 2024-02-01T15:41:11Z | |
dc.date.available | 2024-02-01T15:41:11Z | |
dc.date.issued | 2019-01 | |
dc.description.abstract | Background: A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with specific NK cell characteristics and ImmuKnow® value. Material and Methods: Sixty five subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP. Results: We identified 14 IRP+ [CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (28CD-/CD57+) T cells] individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP- and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition. ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features. Conclusion: NK cells contribute to overall immune senescence in kidney transplant candidates. | |
dc.identifier.issn | 1664-3224 | |
dc.identifier.issn | 1664-3224 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Frontiers Media SA | |
dc.relation.ispartof | Frontiers in immunology | |
dc.relation.isversionof | 10.3389/fimmu.2019.01890 | |
dc.rights.uri | ||
dc.subject | Killer Cells, Natural | |
dc.subject | CD4-Positive T-Lymphocytes | |
dc.subject | CD8-Positive T-Lymphocytes | |
dc.subject | Humans | |
dc.subject | Cytomegalovirus | |
dc.subject | Cytomegalovirus Infections | |
dc.subject | CD4-CD8 Ratio | |
dc.subject | Kidney Transplantation | |
dc.subject | Flow Cytometry | |
dc.subject | Cohort Studies | |
dc.subject | Cross-Sectional Studies | |
dc.subject | Aged | |
dc.subject | Middle Aged | |
dc.subject | Female | |
dc.subject | Male | |
dc.subject | CD57 Antigens | |
dc.title | NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates. | |
dc.type | Journal article | |
duke.contributor.orcid | Jost, Stephanie|0000-0003-2100-3262 | |
pubs.begin-page | 1890 | |
pubs.issue | AUG | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Pathology | |
pubs.organisational-group | Surgery | |
pubs.organisational-group | Surgery, Surgical Sciences | |
pubs.publication-status | Published | |
pubs.volume | 10 |
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