NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates.

dc.contributor.author

DeWolfe, David

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Aid, Malika

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McGann, Kevin

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Ghofrani, Joshua

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Geiger, Emma

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Helzer, Catherine

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Malik, Shaily

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Kleiboeker, Steve

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Jost, Stephanie

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Tan, Chen Sabrina

dc.date.accessioned

2024-02-01T15:41:11Z

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2024-02-01T15:41:11Z

dc.date.issued

2019-01

dc.description.abstract

Background: A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with specific NK cell characteristics and ImmuKnow® value. Material and Methods: Sixty five subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP. Results: We identified 14 IRP+ [CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (28CD-/CD57+) T cells] individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP- and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition. ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features. Conclusion: NK cells contribute to overall immune senescence in kidney transplant candidates.

dc.identifier.issn

1664-3224

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1664-3224

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https://hdl.handle.net/10161/30016

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eng

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Frontiers Media SA

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Frontiers in immunology

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10.3389/fimmu.2019.01890

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https://creativecommons.org/licenses/by-nc/4.0

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Killer Cells, Natural

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CD4-Positive T-Lymphocytes

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CD8-Positive T-Lymphocytes

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Humans

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Cytomegalovirus

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Cytomegalovirus Infections

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CD4-CD8 Ratio

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Kidney Transplantation

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Flow Cytometry

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Cohort Studies

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Cross-Sectional Studies

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Aged

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Middle Aged

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Female

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Male

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CD57 Antigens

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NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates.

dc.type

Journal article

duke.contributor.orcid

Jost, Stephanie|0000-0003-2100-3262

pubs.begin-page

1890

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AUG

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Duke

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School of Medicine

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Clinical Science Departments

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Pathology

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Surgery

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Surgery, Surgical Sciences

pubs.publication-status

Published

pubs.volume

10

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