Quantification of the humidity effect on HR by Ion leakage assay.

dc.contributor.author

Mwimba, Musoki

dc.contributor.author

Dong, Xinnian

dc.date.accessioned

2021-04-01T14:24:41Z

dc.date.available

2021-04-01T14:24:41Z

dc.date.issued

2019-04-05

dc.date.updated

2021-04-01T14:24:41Z

dc.description.abstract

We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the hypersensitive response (HR), the ETI-associated cell death. The method is simple and short enough to handle many biological replicates, which improves the power of test of statistical significance. The protocol is easily applicable to other environmental cues, such as light and temperature, or treatment with chemicals.

dc.identifier

3203

dc.identifier.issn

2331-8325

dc.identifier.issn

2331-8325

dc.identifier.uri

https://hdl.handle.net/10161/22487

dc.language

eng

dc.publisher

Bio-Protocol, LLC

dc.relation.ispartof

Bio-protocol

dc.relation.isversionof

10.21769/bioprotoc.3203

dc.subject

Cell death

dc.subject

ETI

dc.subject

HR

dc.subject

Humidity

dc.subject

Ion leakage

dc.subject

Plant-pathogen interactions

dc.title

Quantification of the humidity effect on HR by Ion leakage assay.

dc.type

Journal article

duke.contributor.orcid

Dong, Xinnian|0000-0002-1120-0951

pubs.begin-page

e3203

pubs.issue

7

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.organisational-group

Biology

pubs.organisational-group

Duke

pubs.publication-status

Published

pubs.volume

9

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Mwimba and Dong Bio-protocol.pdf
Size:
1.07 MB
Format:
Adobe Portable Document Format