Xenon and sevoflurane provide analgesia during labor and fetal brain protection in a perinatal rat model of hypoxia-ischemia.

dc.contributor.author

Yang, Ting

dc.contributor.author

Zhuang, Lei

dc.contributor.author

Rei Fidalgo, António M

dc.contributor.author

Petrides, Evgenia

dc.contributor.author

Terrando, Niccolo

dc.contributor.author

Wu, Xinmin

dc.contributor.author

Sanders, Robert D

dc.contributor.author

Robertson, Nicola J

dc.contributor.author

Johnson, Mark R

dc.contributor.author

Maze, Mervyn

dc.contributor.author

Ma, Daqing

dc.contributor.editor

Landau, Ruth

dc.date.accessioned

2018-04-10T19:42:34Z

dc.date.available

2018-04-10T19:42:34Z

dc.date.issued

2012-01

dc.date.updated

2018-04-10T19:42:32Z

dc.description.abstract

It is not possible to identify all pregnancies at risk of neonatal hypoxic-ischemic encephalopathy (HIE). Many women use some form of analgesia during childbirth and some anesthetic agents have been shown to be neuroprotective when used as analgesics at subanesthetic concentrations. In this study we sought to understand the effects of two anesthetic agents with presumptive analgesic activity and known preconditioning-neuroprotective properties (sevoflurane or xenon), in reducing hypoxia-induced brain damage in a model of intrauterine perinatal asphyxia. The analgesic and neuroprotective effects at subanesthetic levels of sevoflurane (0.35%) or xenon (35%) were tested in a rat model of intrauterine perinatal asphyxia. Analgesic effects were measured by assessing maternal behavior and spinal cord dorsal horn neuronal activation using c-Fos. In separate experiments, intrauterine fetal asphyxia was induced four hours after gas exposure; on post-insult day 3 apoptotic cell death was measured by caspase-3 immunostaining in hippocampal neurons and correlated with the number of viable neurons on postnatal day (PND) 7. A separate cohort of pups was nurtured by a surrogate mother for 50 days when cognitive testing with Morris water maze was performed. Both anesthetic agents provided analgesia as reflected by a reduction in the number of stretching movements and decreased c-Fos expression in the dorsal horn of the spinal cord. Both agents also reduced the number of caspase-3 positive (apoptotic) neurons and increased cell viability in the hippocampus at PND7. These acute histological changes were mirrored by improved cognitive function measured remotely after birth on PND 50 compared to control group. Subanesthetic doses of sevoflurane or xenon provided both analgesia and neuroprotection in this model of intrauterine perinatal asphyxia. These data suggest that anesthetic agents with neuroprotective properties may be effective in preventing HIE and should be tested in clinical trials in the future.

dc.identifier.issn

1932-6203

dc.identifier.issn

1932-6203

dc.identifier.uri

https://hdl.handle.net/10161/16496

dc.language

eng

dc.publisher

Public Library of Science (PLoS)

dc.relation.ispartof

PloS one

dc.relation.isversionof

10.1371/journal.pone.0037020

dc.subject

Brain

dc.subject

Hippocampus

dc.subject

Posterior Horn Cells

dc.subject

Animals

dc.subject

Animals, Newborn

dc.subject

Rats

dc.subject

Rats, Sprague-Dawley

dc.subject

Hypoxia-Ischemia, Brain

dc.subject

Memory Disorders

dc.subject

Labor Pain

dc.subject

Xenon

dc.subject

Methyl Ethers

dc.subject

Proto-Oncogene Proteins c-fos

dc.subject

Neuroprotective Agents

dc.subject

Analgesia

dc.subject

Cohort Studies

dc.subject

Apoptosis

dc.subject

Cell Survival

dc.subject

Pregnancy

dc.subject

Female

dc.subject

Fetal Hypoxia

dc.subject

Caspase 3

dc.title

Xenon and sevoflurane provide analgesia during labor and fetal brain protection in a perinatal rat model of hypoxia-ischemia.

dc.type

Journal article

duke.contributor.orcid

Yang, Ting|0000-0002-5113-8611

duke.contributor.orcid

Terrando, Niccolo|0000-0003-1803-5853

pubs.issue

5

pubs.organisational-group

School of Medicine

pubs.organisational-group

Duke

pubs.organisational-group

Cell Biology

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Duke Institute for Brain Sciences

pubs.organisational-group

University Institutes and Centers

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.organisational-group

Anesthesiology

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Medicine, Nephrology

pubs.organisational-group

Medicine

pubs.publication-status

Published

pubs.volume

7

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Xenon and sevoflurane provide analgesia during labor and fetal brain protection in a perinatal rat model of hypoxia-ischemia.pdf
Size:
634.06 KB
Format:
Adobe Portable Document Format