Wood smoke particle exposure in mice reduces the severity of influenza infection.

dc.contributor.author

Vose, Aaron

dc.contributor.author

McCravy, Matthew

dc.contributor.author

Birukova, Anastasiya

dc.contributor.author

Yang, Zhonghui

dc.contributor.author

Hollingsworth, John W

dc.contributor.author

Que, Loretta G

dc.contributor.author

Tighe, Robert M

dc.date.accessioned

2024-07-16T16:30:52Z

dc.date.available

2024-07-16T16:30:52Z

dc.date.issued

2021-09

dc.description.abstract

Elevated ambient temperatures and extreme weather events have increased the incidence of wildfires world-wide resulting in increased wood smoke particle (WSP). Epidemiologic data suggests that WSP exposure associates with exacerbations of respiratory diseases, and with increased respiratory viral infections. To assess the impact of WSP exposure on host response to viral pneumonia, we performed WSP exposures in rodents followed by infection with mouse adapted influenza (HINI-PR8). C57BL/6 male mice aged 6-8 weeks were challenged with WSP or PBS by oropharyngeal aspiration in acute (single dose) or sub-acute exposures (day 1, 3, 5, 7 and 10). Additional groups underwent sub-acute exposure followed by infection by influenza or heat-inactivated (HI) virus. Following exposures/infection, bronchoalveolar lavage (BAL) was performed to assess for total cell counts/differentials, total protein, protein carbonyls and hyaluronan. Lung tissue was assessed for viral counts by real time PCR. When compared to PBS, acute WSP exposure associated with an increase in airspace macrophages. Alternatively, sub-acute exposure resulted in a dose dependent increase in airspace neutrophils. Sub-acute WSP exposure followed by influenza infection was associated with improved respiratory viral outcomes including reduced weight loss and increased blood oxygen saturation, and decreased protein carbonyls and viral titers. Flow cytometry demonstrated dynamic changes in pulmonary macrophage and T cell subsets based on challenge with WSP and influenza. This data suggests that sub-acute WSP exposure can improve host response to acute influenza infection.

dc.identifier

S0041-008X(21)00249-0

dc.identifier.issn

0041-008X

dc.identifier.issn

1096-0333

dc.identifier.uri

https://hdl.handle.net/10161/31279

dc.language

eng

dc.publisher

Elsevier BV

dc.relation.ispartof

Toxicology and applied pharmacology

dc.relation.isversionof

10.1016/j.taap.2021.115645

dc.rights.uri

https://creativecommons.org/licenses/by-nc/4.0

dc.subject

Lung

dc.subject

Macrophages

dc.subject

Animals

dc.subject

Mice, Inbred C57BL

dc.subject

Mice

dc.subject

Pneumonia, Viral

dc.subject

Orthomyxoviridae Infections

dc.subject

Administration, Inhalation

dc.subject

Severity of Illness Index

dc.subject

Smoke

dc.subject

Virus Replication

dc.subject

Wood

dc.subject

Male

dc.subject

Influenza A Virus, H1N1 Subtype

dc.subject

Transcriptome

dc.subject

Wildfires

dc.title

Wood smoke particle exposure in mice reduces the severity of influenza infection.

dc.type

Journal article

duke.contributor.orcid

Vose, Aaron|0000-0003-2745-7070

duke.contributor.orcid

McCravy, Matthew|0000-0001-5313-211X

duke.contributor.orcid

Tighe, Robert M|0000-0002-3465-9861

pubs.begin-page

115645

pubs.organisational-group

Duke

pubs.organisational-group

Nicholas School of the Environment

pubs.organisational-group

School of Medicine

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Medicine

pubs.organisational-group

Medicine, Pulmonary, Allergy, and Critical Care Medicine

pubs.organisational-group

Environmental Sciences and Policy

pubs.publication-status

Published

pubs.volume

426

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Wood smoke particle exposure in mice reduces the severity of influenza infection.pdf
Size:
1.3 MB
Format:
Adobe Portable Document Format