NLP7 PB1 Domain Interactions and Condensation Regulate the Plant Nitrogen Response

Abstract

Nitrogen is an essential element for all organisms as a crucial component of nucleic and amino acids. The NIN-LIKE PROTEINS (NLPs) are the central transcription factors (TFs) regulating the primary nitrate response (PNR) in plants. In Arabidopsis thaliana, NLP7 is the master regulator of the PNR, regulating the expression of thousands of nitrate responsive genes. The mechanisms governing Here I investigate the role of PB1 domain interactions in the biological function of NLP7 and how nucleocytoplasmic partitioning of NLP7 contributes to the plant nitrate response. Additionally, I compare the structure and mechanisms regulating NLP7 to the AUXIN RESPONSE FACTORS (ARFs) which are also DNA-binding transcription factors with PB1 domains and large IDRs, that undergo nucleocytoplasmic partitioning.