Cannabinoid exposure and altered DNA methylation in rat and human sperm.

dc.contributor.author

Murphy, Susan K

dc.contributor.author

Itchon-Ramos, Nilda

dc.contributor.author

Visco, Zachary

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Huang, Zhiqing

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Grenier, Carole

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Schrott, Rose

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Acharya, Kelly

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Boudreau, Marie-Helene

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Price, Thomas M

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Raburn, Douglas J

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Corcoran, David L

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Lucas, Joseph E

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Mitchell, John T

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McClernon, F Joseph

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Cauley, Marty

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Hall, Brandon J

dc.contributor.author

Levin, Edward D

dc.contributor.author

Kollins, Scott H

dc.date.accessioned

2019-04-21T21:55:41Z

dc.date.available

2019-04-21T21:55:41Z

dc.date.issued

2018-01

dc.date.updated

2019-04-21T21:55:38Z

dc.description.abstract

Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measured by reduced representation bisulfite sequencing, differed in the sperm of human users from non-users by at least 10% at 3,979 CpG sites. Pathway analyses indicated Hippo Signaling and Pathways in Cancer as enriched with altered genes (Bonferroni p < 0.02). These same two pathways were also enriched with genes having altered methylation in sperm from THC-exposed versus vehicle-exposed rats (p < 0.01). Data validity is supported by significant correlations between THC exposure levels in humans and methylation for 177 genes, and substantial overlap in THC target genes in rat sperm (this study) and genes previously reported as having altered methylation in the brain of rat offspring born to parents both exposed to THC during adolescence. In humans, cannabis use was also associated with significantly lower sperm concentration. Findings point to possible pre-conception paternal reproductive risks associated with cannabis use.

dc.identifier.issn

1559-2294

dc.identifier.issn

1559-2308

dc.identifier.uri

https://hdl.handle.net/10161/18357

dc.language

eng

dc.publisher

Informa UK Limited

dc.relation.ispartof

Epigenetics

dc.relation.isversionof

10.1080/15592294.2018.1554521

dc.subject

Cannabis

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DNA methylation

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epigenetic reprogramming

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human

dc.subject

rat

dc.subject

sperm

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tetrahydrocannabinol

dc.title

Cannabinoid exposure and altered DNA methylation in rat and human sperm.

dc.type

Journal article

duke.contributor.orcid

Murphy, Susan K|0000-0001-8298-7272

duke.contributor.orcid

Acharya, Kelly|0000-0003-1891-4196

duke.contributor.orcid

Price, Thomas M|0000-0003-1888-306X

duke.contributor.orcid

Raburn, Douglas J|0000-0002-7984-1245

duke.contributor.orcid

Mitchell, John T|0000-0002-9586-3823

duke.contributor.orcid

McClernon, F Joseph|0000-0002-2846-980X

duke.contributor.orcid

Levin, Edward D|0000-0001-7292-8084|0000-0002-5060-9602

duke.contributor.orcid

Kollins, Scott H|0000-0001-6847-6935

pubs.begin-page

1208

pubs.end-page

1221

pubs.issue

12

pubs.organisational-group

School of Medicine

pubs.organisational-group

Duke

pubs.organisational-group

Psychology and Neuroscience

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.organisational-group

Center for Child and Family Policy

pubs.organisational-group

Sanford School of Public Policy

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Duke Cancer Institute

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Institutes and Centers

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Duke Clinical Research Institute

pubs.organisational-group

Duke Institute for Brain Sciences

pubs.organisational-group

University Institutes and Centers

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.organisational-group

Psychiatry & Behavioral Sciences, Addictions

pubs.organisational-group

Psychiatry & Behavioral Sciences

pubs.organisational-group

Clinical Science Departments

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Environmental Sciences and Policy

pubs.organisational-group

Nicholas School of the Environment

pubs.organisational-group

Pathology

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Obstetrics and Gynecology, Reproductive Endocrinology & Fertility

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Obstetrics and Gynecology

pubs.organisational-group

Duke-UNC Center for Brain Imaging and Analysis

pubs.organisational-group

Faculty

pubs.organisational-group

Staff

pubs.publication-status

Published

pubs.volume

13

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