Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of <i>Caenorhabditis elegans</i>.
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2021-03
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Abstract
Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).
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Hibshman, Jonathan D, Amy K Webster and L Ryan Baugh (2021). Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans. STAR protocols, 2(1). p. 100276. 10.1016/j.xpro.2020.100276 Retrieved from https://hdl.handle.net/10161/22273.
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L. Ryan Baugh
The Baugh Lab is interested in phenotypic plasticity and developmental robustness despite variable environmental conditions. We use the roundworm C. elegans to study how animals adapt to starvation over different time scales using functional genomics (bulk and single-cell) as well as statistical, quantitative, and molecular genetics. Our research questions revolve around how gene regulation and development are governed by nutrient availability, how animals acclimate to survive starvation, and the mechanisms underlying adult consequences of early life starvation. We are gaining insight into the genetic basis of natural variation among wild strains, the function of conserved tumor suppressors, epigenetic effects of starvation, and how early life experience affects adult disease.
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