Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of <i>Caenorhabditis elegans</i>.

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2021-03

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Abstract

Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).

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10.1016/j.xpro.2020.100276

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Hibshman, Jonathan D, Amy K Webster and L Ryan Baugh (2021). Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans. STAR protocols, 2(1). p. 100276. 10.1016/j.xpro.2020.100276 Retrieved from https://hdl.handle.net/10161/22273.

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Scholars@Duke

Baugh

L. Ryan Baugh

Professor of Biology

The Baugh Lab is interested in phenotypic plasticity and physiological adaptation to variable environmental conditions. We are using the roundworm C. elegans to understand how animals adapt to starvation using primarily genetic and genomic approaches. We are studying how development is governed by nutrient availability, how animals survive starvation, and the long-term consequences of starvation including adult disease and transgenerational epigenetic inheritance.


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