Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of <i>Caenorhabditis elegans</i>.

dc.contributor.author

Hibshman, Jonathan D

dc.contributor.author

Webster, Amy K

dc.contributor.author

Baugh, L Ryan

dc.date.accessioned

2021-02-01T14:23:49Z

dc.date.available

2021-02-01T14:23:49Z

dc.date.issued

2021-03

dc.date.updated

2021-02-01T14:23:46Z

dc.description.abstract

Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).

dc.identifier

S2666-1667(20)30263-X

dc.identifier.issn

2666-1667

dc.identifier.issn

2666-1667

dc.identifier.uri

https://hdl.handle.net/10161/22273

dc.language

eng

dc.publisher

Elsevier BV

dc.relation.ispartof

STAR protocols

dc.relation.isversionof

10.1016/j.xpro.2020.100276

dc.subject

Cell biology

dc.subject

Genetics

dc.subject

Model organisms

dc.title

Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans.

dc.type

Journal article

duke.contributor.orcid

Baugh, L Ryan|0000-0003-2148-5492

pubs.begin-page

100276

pubs.issue

1

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.organisational-group

Biology

pubs.organisational-group

Duke

pubs.publication-status

Accepted

pubs.volume

2

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