Snap-shot multispectral imaging of vascular dynamics in a mouse window-chamber model.

Hendargo, Hansford C

Zhao, Yulin

Allenby, Taylor

Palmer, Gregory M

United States

2015-07-28T14:09:37Z

2015-07-15

Understanding tumor vascular dynamics through parameters such as blood flow and oxygenation can yield insight into tumor biology and therapeutic response. Hyperspectral microscopy enables optical detection of hemoglobin saturation or blood velocity by either acquiring multiple images that are spectrally distinct or by rapid acquisition at a single wavelength over time. However, the serial acquisition of spectral images over time prevents the ability to monitor rapid changes in vascular dynamics and cannot monitor concurrent changes in oxygenation and flow rate. Here, we introduce snap shot-multispectral imaging (SS-MSI) for use in imaging the microvasculature in mouse dorsal-window chambers. By spatially multiplexing spectral information into a single-image capture, simultaneous acquisition of dynamic hemoglobin saturation and blood flow over time is achieved down to the capillary level and provides an improved optical tool for monitoring rapid in vivo vascular dynamics.

http://www.ncbi.nlm.nih.gov/pubmed/26176452

321650

1539-4794

https://hdl.handle.net/10161/10324

eng

The Optical Society

Opt Lett

Animals

Hemoglobins

Mice

Mice, Inbred C57BL

Microvessels

Molecular Imaging

Snap-shot multispectral imaging of vascular dynamics in a mouse window-chamber model.

Journal article

Palmer, Gregory M|0000-0003-2955-8297

http://www.ncbi.nlm.nih.gov/pubmed/26176452

3292

3295

14

Clinical Science Departments

Duke

Duke Cancer Institute

Institutes and Centers

Radiation Oncology

School of Medicine

Published

40