Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds.

dc.contributor.author

Abrahamsson, Christoffer K

dc.contributor.author

Yang, Fan

dc.contributor.author

Park, Hyoungshin

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Brunger, Jonathan M

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Valonen, Piia K

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Langer, Robert

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Welter, Jean F

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Caplan, Arnold I

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Guilak, Farshid

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Freed, Lisa E

dc.coverage.spatial

United States

dc.date.accessioned

2011-04-15T16:46:37Z

dc.date.issued

2010-12

dc.description.abstract

Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven poly(ɛ-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue engineering. We hypothesized that in vitro culture duration and medium additives can individually and interactively influence the structure, composition, mechanical, and molecular properties of engineered tissues based on hMSCs and 3D poly(ɛ-caprolactone). Bone marrow hMSCs were suspended in collagen gel, seeded on scaffolds, and cultured for 1, 21, or 45 days under chondrogenic and/or osteogenic conditions. Structure, composition, biomechanics, and gene expression were analyzed. In chondrogenic medium, cartilaginous tissue formed by day 21, and hypertrophic mineralization was observed in the newly formed extracellular matrix at the interface with underlying scaffold by day 45. Glycosaminoglycan, hydroxyproline, and calcium contents, and alkaline phosphatase activity depended on culture duration and medium additives, with significant interactive effects (all p < 0.0001). The 45-day constructs exhibited mechanical properties on the order of magnitude of native articular cartilage (aggregate, Young's, and shear moduli of 0.15, 0.12, and 0.033 MPa, respectively). Gene expression was characteristic of chondrogenesis and endochondral bone formation, with sequential regulation of Sox-9, collagen type II, aggrecan, core binding factor alpha 1 (Cbfα1)/Runx2, bone sialoprotein, bone morphogenetic protein-2, and osteocalcin. In contrast, osteogenic medium produced limited osteogenesis. Long-term culture of hMSC on 3D scaffolds resulted in chondrogenesis and regional mineralization at the interface between soft, newly formed engineered cartilage, and stiffer underlying scaffold. These findings merit consideration when developing grafts for osteochondral defect repair.

dc.description.version

Version of Record

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/20673022

dc.identifier.eissn

1937-335X

dc.identifier.uri

https://hdl.handle.net/10161/3347

dc.language

eng

dc.language.iso

en_US

dc.publisher

Mary Ann Liebert Inc

dc.relation.ispartof

Tissue Eng Part A

dc.relation.isversionof

10.1089/ten.TEA.2010.0190

dc.relation.journal

Tissue Engineering Part a

dc.subject

Cells, Cultured

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Chondrogenesis

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Humans

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Male

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Mesenchymal Stromal Cells

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Middle Aged

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Tissue Engineering

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Tissue Scaffolds

dc.title

Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds.

dc.type

Journal article

duke.date.pubdate

2010-12-0

duke.description.issue

12

duke.description.volume

16

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/20673022

pubs.begin-page

3709

pubs.end-page

3718

pubs.issue

12

pubs.organisational-group

Biomedical Engineering

pubs.organisational-group

Duke

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Pratt School of Engineering

pubs.publication-status

Published

pubs.volume

16

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