Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment.
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2024-03
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Abstract
Spinal column tumors can be difficult to process for single-cell omic studies, given the heterogeneity in tissue. Here, we present a protocol for operating room-to-benchtop single-cell processing of clinical specimens from a prostate cancer patient. We describe steps for sample homogenization, red blood cell lysis, cryopreservation, and single-cell sequencing analysis. This protocol can be used to identify prognostic markers and therapeutic targets for patients with osseous spine metastases and better inform eligibility for clinical trials.
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Nguyen, Annee D, Corinne Haines, Meghan J Price, Tara E Dalton, César D Baëta, Harrison A Hockenberry and C Rory Goodwin (2024). Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment. STAR protocols, 5(1). p. 102805. 10.1016/j.xpro.2023.102805 Retrieved from https://hdl.handle.net/10161/33173.
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Annee Nguyen
Annee is a graduate student in Duke's Department of Pharmacology, a joint program with Molecular Cancer Biology. She is excited to identify therapeutic molecules and elucidate therapeutic mechanisms of antitumor efficacy that may also target secondary comorbid conditions, such as pain or neurological deficits. She is passionate about scientific education and communication and hopes to remain in academia with the goal of cultivating the next generation of scientists.
In 2017, Annee graduated from the University of California, San Diego, from Warren College, with a Bachelor of Science in Human Biology (double minoring in Psychology and Theatre) as magna cum laude and as part of the Sigma Chapter of the Phi Beta Kappa Society and obtained a contiguous Master of Science in Biology in 2018, concentrating in Neuropathology and Scientific Communication.
Since 2014, her prior research experience includes working with various preclinical models of peripheral neuropathy (with Dr. Nigel Calcutt and Dr. Christina Sigurdson of UC San Diego’s Department of Pathology), equine and murine models of neuroaxonal dystrophy (with Dr. Carrie Finno of UC Davis’s School of Veterinary Medicine), and CNS tumor preclinical models of recurrent glioblastoma and spine tumors and metastases at Duke University Medical Center with Dr. Rory Goodwin of the Duke Center for Brain and Spine Metastasis and the Department of Neurosurgery. In each lab, she sought to identify therapeutics that could prevent, reverse, or abate the neurodegenerative diseases of focus and explored mechanisms of disease development and therapeutic impact. Throughout her experiences, Annee has continually focused on developing projects driven by clinical impact and focused on taking science from bench to bedside and back.
Courtney Rory Goodwin
Associate Professor of Neurosurgery, Radiation Oncology, Orthopedic Surgery.
Director of Spine Oncology,
Associate Residency Program Director
Third Year Study Program Director Neurosciences, Duke University School of Medicine
Director of Spine Metastasis, Duke Center for Brain and Spine Metastasis, Department of Neurosurgery
Duke Cancer Institute, Duke University Medical Center
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