Myocardial Strain and Cardiac Output are Preferable Measurements for Cardiac Dysfunction and Can Predict Mortality in Septic Mice.

dc.contributor.author

Hoffman, Matthew

dc.contributor.author

Kyriazis, Ioannis D

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Lucchese, Anna M

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de Lucia, Claudio

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Piedepalumbo, Michela

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Bauer, Michael

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Schulze, P Christian

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Bonios, Michael J

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Koch, Walter J

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Drosatos, Konstantinos

dc.date.accessioned

2024-11-14T23:19:40Z

dc.date.available

2024-11-14T23:19:40Z

dc.date.issued

2019-05

dc.description.abstract

Background Sepsis is the overwhelming host response to infection leading to shock and multiple organ dysfunction. Cardiovascular complications greatly increase sepsis-associated mortality. Although murine models are routinely used for preclinical studies, the benefit of using genetically engineered mice in sepsis is countered by discrepancies between human and mouse sepsis pathophysiology. Therefore, recent guidelines have called for standardization of preclinical methods to document organ dysfunction. We investigated the course of cardiac dysfunction and myocardial load in different mouse models of sepsis to identify the optimal measurements for early systolic and diastolic dysfunction. Methods and Results We performed speckle-tracking echocardiography and assessed blood pressure, plasma inflammatory cytokines, lactate, B-type natriuretic peptide, and survival in mouse models of endotoxemia or polymicrobial infection (cecal ligation and puncture, [ CLP ]) of moderate and high severity. We observed that myocardial strain and cardiac output were consistently impaired early in both sepsis models. Suppression of cardiac output was associated with systolic dysfunction in endotoxemia or combined systolic dysfunction and reduced preload in the CLP model. We found that cardiac output at 2 hours post- CLP is a negative prognostic indicator with high sensitivity and specificity that predicts mortality at 48 hours. Using a known antibiotic (ertapenem) treatment, we confirmed that this approach can document recovery. Conclusions We propose a non-invasive approach for assessment of cardiac function in sepsis and myocardial strain and strain rate as preferable measures for monitoring cardiovascular function in sepsis mouse models. We further show that the magnitude of cardiac output suppression 2 hours post- CLP can be used to predict mortality.

dc.identifier.issn

2047-9980

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2047-9980

dc.identifier.uri

https://hdl.handle.net/10161/31649

dc.language

eng

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Ovid Technologies (Wolters Kluwer Health)

dc.relation.ispartof

Journal of the American Heart Association

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10.1161/jaha.119.012260

dc.rights.uri

https://creativecommons.org/licenses/by-nc/4.0

dc.subject

Animals

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Mice, Inbred C57BL

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Sepsis

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Cardiomyopathies

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Disease Models, Animal

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Disease Progression

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Lactic Acid

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Natriuretic Peptide, Brain

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Inflammation Mediators

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Cytokines

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Echocardiography, Doppler

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Cardiac Output

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Risk Factors

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Predictive Value of Tests

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Myocardial Contraction

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Ventricular Function, Left

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Time Factors

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Male

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Biomarkers

dc.title

Myocardial Strain and Cardiac Output are Preferable Measurements for Cardiac Dysfunction and Can Predict Mortality in Septic Mice.

dc.type

Journal article

duke.contributor.orcid

Koch, Walter J|0000-0002-8522-530X

pubs.begin-page

e012260

pubs.issue

10

pubs.organisational-group

Duke

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School of Medicine

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Clinical Science Departments

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Surgery

pubs.organisational-group

Surgery, Cardiovascular and Thoracic Surgery

pubs.publication-status

Published

pubs.volume

8

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