In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.
Date
2014-03
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Abstract
Background
Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro.New method
Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo.Results
We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage.Conclusions
The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.Type
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Madison, Roger D, Grant A Robinson, Christian Krarup, Mihai Moldovan, Qiang Li and Wilkie A Wilson (2014). In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation. Journal of neuroscience methods, 225. pp. 90–96. 10.1016/j.jneumeth.2014.01.018 Retrieved from https://hdl.handle.net/10161/34000.
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Scholars@Duke
Roger D. Madison
Neuronal Plasticity: especially as related to the accuracy of peripheral nerve regeneration. My laboratory is currently involved in studying the efficacy of prosthetic "nerve guides" in rodents and non-human primates. The results suggest that such nerve guides can be as effective as a nerve graft to repair transected peripheral nerves. Limited clinical trials of the nerve guide prostheses are underway, in collaboration with a colleague in Denmark. The nerve regeneration work has more recently taken a molecular turn, and my laboratory is currently looking at the differential expression of genes that may underlie the accuracy of peripheral nerve regeneration. We have developed a double labeling technique which allows us to assess the accuracy of nerve regeneration at the single neuron level. We are finding that motor axons and sensory afferents to muscle display a greater than chance level to grow back to muscle as opposed to skin (ie. regeneration specificity). To identify genes and gene products that may be involved in this process, we are using classical subtractive hybridization, the PCR-based differential display of mRNAs, and amplified antisense RNA (aRNA) for Êexpression profilingË.
Grant Alan Robinson
My research interests are in central and peripheral nervous system regeneration.
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