A role of the CTCF binding site at enhancer Eα in the dynamic chromatin organization of the Tcra-Tcrd locus.

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2020-09

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Abstract

The regulation of T cell receptor Tcra gene rearrangement has been extensively studied. The enhancer Eα plays an essential role in Tcra rearrangement by establishing a recombination centre in the Jα array and a chromatin hub for interactions between Vα and Jα genes. But the mechanism of the Eα and its downstream CTCF binding site (here named EACBE) in dynamic chromatin regulation is unknown. The Hi-C data showed that the EACBE is located at the sub-TAD boundary which separates the Tcra-Tcrd locus and the downstream region including the Dad1 gene. The EACBE is required for long-distance regulation of the Eα on the proximal Vα genes, and its deletion impaired the Tcra rearrangement. We also noticed that the EACBE and Eα regulate the genes in the downstream sub-TAD via asymmetric chromatin extrusion. This study provides a new insight into the role of CTCF binding sites at TAD boundaries in gene regulation.

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Thymus Gland, Chromatin, Animals, Mice, Inbred C57BL, Mice, Mutant Strains, Homeodomain Proteins, Membrane Proteins, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Antigen, T-Cell, gamma-delta, Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, Gene Expression Regulation, Binding Sites, Apoptosis Regulatory Proteins, Enhancer Elements, Genetic, CCCTC-Binding Factor

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https://hdl.handle.net/10161/30472

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https://creativecommons.org/licenses/by-nc/4.0

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10.1093/nar/gkaa711

Publication Info

Zhao, Hao, Zhaoqiang Li, Yongchang Zhu, Shasha Bian, Yan Zhang, Litao Qin, Abani Kanta Naik, Jiangtu He, et al. (2020). A role of the CTCF binding site at enhancer Eα in the dynamic chromatin organization of the Tcra-Tcrd locus. Nucleic acids research, 48(17). pp. 9621–9636. 10.1093/nar/gkaa711 Retrieved from https://hdl.handle.net/10161/30472.

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