Patterning Mechanisms Underlying Notochord and Spine Segmentation in Zebrafish

dc.contributor.advisor

Bagnat, Michel

dc.contributor.author

Wopat, Susan

dc.date.accessioned

2021-05-19T18:08:27Z

dc.date.available

2023-05-17T08:17:10Z

dc.date.issued

2021

dc.department

Cell Biology

dc.description.abstract

The defining characteristic of the subphylum Vertebrata is the vertebral column, which is comprised of alternating vertebral bodies and intervertebral discs. In spite of being a highly conserved structure, the morphogenetic events that culminate in building the vertebral column remain poorly understood. In particular, patterning mechanisms underlying how segmentation of the spine is precisely established have not been examined at post-embryonic stages. For several years, vertebral column patterning was thought to hinge upon proper segmentation of the embryo, while the notochord served as a transient scaffold for the vertebral bodies and intervertebral discs. Using genetic, live-imaging, and quantitative approaches, this work illustrates that the notochord sheath in zebrafish, provides a template for osteoblast recruitment and vertebral bone formation in the developing spine. Furthermore, we show that notochord segmentation is influenced by the adjacent muscle segments and connective tissue, which may provide mechanical patterning cues. Insights from this work will better inform how adolescent idiopathic scoliosis and congenital scoliosis arise.

dc.identifier.uri

https://hdl.handle.net/10161/23050

dc.subject

Cellular biology

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Developmental biology

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Morphology

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intervertebral disc

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notochord

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segmentation

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spine

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vertebrae

dc.title

Patterning Mechanisms Underlying Notochord and Spine Segmentation in Zebrafish

dc.type

Dissertation

duke.embargo.months

23.9013698630137

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