Development and evaluation of a real-time polymerase chain reaction assay for the rapid detection of Talaromyces marneffei MP1 gene in human plasma
| dc.contributor.author | Hien, Ha Thuc Ai | |
| dc.contributor.author | Thanh, Tran Tan | |
| dc.contributor.author | Thu, Nguyen Thi Mai | |
| dc.contributor.author | Nguyen, Ashley | |
| dc.contributor.author | Thanh, Nguyen Tat | |
| dc.contributor.author | Lan, Nguyen Phu Huong | |
| dc.contributor.author | Simmons, Cameron | |
| dc.contributor.author | Shikuma, Cecilia | |
| dc.contributor.author | Chau, Nguyen Van Vinh | |
| dc.contributor.author | Thwaites, Guy | |
| dc.contributor.author | Le, Thuy | |
| dc.date.accessioned | 2018-03-16T19:34:28Z | |
| dc.date.available | 2018-03-16T19:34:28Z | |
| dc.date.issued | 2016-12-01 | |
| dc.description.abstract | © 2016 The Authors. Mycoses Published by Blackwell Verlag GmbH Penicilliosis caused by Talaromyces marneffei is a common AIDS-defining illness in South and Southeast Asia. Diagnosis is based on culture which can take up to 14 days for identification, leading to treatment delay and increased mortality. We developed a TaqMan real-time PCR assay targeting the MP1 gene encoding an abundant cell wall protein specific to T. marneffei. The assay's performance was evaluated in MP1-containing plasmids, clinical isolates, and plasma from HIV-infected patients with and without penicilliosis. The assay consistently detected 10 copies of MP1-containing plasmids per reaction and 100 T. marneffei yeast cells per millilitre plasma. There were no amplification with seven other Penicillium species and six other HIV-associated fungal pathogens tested. The assay was evaluated in 70 patients with AIDS: 50 patients with culture-confirmed penicilliosis and 20 patients with opportunistic infections other than penicilliosis. The diagnostic sensitivity was 70.4% (19/27, 95% CI: 51.5–84.1%) and 52.2% (12/23, 95% CI: 33.0–70.8%) in plasma samples collected prior to and within 48 h of antifungal therapy respectively. The diagnostic specificity was 100% (20/20, 95% CI: 83.9–100%). This assay provides a useful tool for the rapid diagnosis of T. marneffei infection and has the potential to improve the management of patients with penicilliosis. | |
| dc.identifier.eissn | 1439-0507 | |
| dc.identifier.issn | 0933-7407 | |
| dc.identifier.uri | ||
| dc.publisher | Wiley | |
| dc.relation.ispartof | Mycoses | |
| dc.relation.isversionof | 10.1111/myc.12530 | |
| dc.title | Development and evaluation of a real-time polymerase chain reaction assay for the rapid detection of Talaromyces marneffei MP1 gene in human plasma | |
| dc.type | Journal article | |
| duke.contributor.orcid | Le, Thuy|0000-0002-3393-6580 | |
| pubs.begin-page | 773 | |
| pubs.end-page | 780 | |
| pubs.issue | 12 | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Medicine, Infectious Diseases | |
| pubs.organisational-group | School of Medicine | |
| pubs.publication-status | Published | |
| pubs.volume | 59 |
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