Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.


Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.






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Publication Info

Goodwin, C Rory, Crystal L Woodard, Xin Zhou, Jianbo Pan, Alessandro Olivi, Shuli Xia, Chetan Bettegowda, Daniel M Sciubba, et al. (2016). Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems. Transl Oncol, 9(2). pp. 124–129. 10.1016/j.tranon.2016.02.001 Retrieved from https://hdl.handle.net/10161/15820.

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Courtney Rory Goodwin

Associate Professor of Neurosurgery

Associate Professor of Neurosurgery, Radiation Oncology, Orthopedic Surgery.
Director of Spine Oncology,
Associate Residency Program Director
Third Year Study Program Director Neurosciences, Duke University School of Medicine
Director of Spine Metastasis, Duke Center for Brain and Spine Metastasis, Department of Neurosurgery
Duke Cancer Institute, Duke University Medical Center

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