Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

dc.contributor.author

Goodwin, C Rory

dc.contributor.author

Woodard, Crystal L

dc.contributor.author

Zhou, Xin

dc.contributor.author

Pan, Jianbo

dc.contributor.author

Olivi, Alessandro

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Xia, Shuli

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Bettegowda, Chetan

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Sciubba, Daniel M

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Pevsner, Jonathan

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Zhu, Heng

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Laterra, John

dc.coverage.spatial

United States

dc.date.accessioned

2017-12-02T17:20:02Z

dc.date.available

2017-12-02T17:20:02Z

dc.date.issued

2016-04

dc.description.abstract

Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/27084428

dc.identifier

S1936-5233(15)30053-X

dc.identifier.issn

1936-5233

dc.identifier.uri

https://hdl.handle.net/10161/15820

dc.language

eng

dc.publisher

Elsevier BV

dc.relation.ispartof

Transl Oncol

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10.1016/j.tranon.2016.02.001

dc.title

Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

dc.type

Journal article

duke.contributor.orcid

Goodwin, C Rory|0000-0002-6540-2751

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/27084428

pubs.begin-page

124

pubs.end-page

129

pubs.issue

2

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Duke

pubs.organisational-group

Neurosurgery

pubs.organisational-group

School of Medicine

pubs.organisational-group

Temp group - logins allowed

pubs.publication-status

Published

pubs.volume

9

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