Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.

dc.contributor.author

Sheard, Laura B

dc.contributor.author

Tan, Xu

dc.contributor.author

Mao, Haibin

dc.contributor.author

Withers, John

dc.contributor.author

Ben-Nissan, Gili

dc.contributor.author

Hinds, Thomas R

dc.contributor.author

Kobayashi, Yuichi

dc.contributor.author

Hsu, Fong-Fu

dc.contributor.author

Sharon, Michal

dc.contributor.author

Browse, John

dc.contributor.author

He, Sheng Yang

dc.contributor.author

Rizo, Josep

dc.contributor.author

Howe, Gregg A

dc.contributor.author

Zheng, Ning

dc.date.accessioned

2020-11-25T23:15:38Z

dc.date.available

2020-11-25T23:15:38Z

dc.date.issued

2010-11

dc.date.updated

2020-11-25T23:15:35Z

dc.description.abstract

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

dc.identifier

nature09430

dc.identifier.issn

0028-0836

dc.identifier.issn

1476-4687

dc.identifier.uri

https://hdl.handle.net/10161/21727

dc.language

eng

dc.publisher

Springer Science and Business Media LLC

dc.relation.ispartof

Nature

dc.relation.isversionof

10.1038/nature09430

dc.subject

Arabidopsis

dc.subject

Inositol Phosphates

dc.subject

Cyclopentanes

dc.subject

Indenes

dc.subject

Plant Growth Regulators

dc.subject

Amino Acids

dc.subject

Isoleucine

dc.subject

Peptide Fragments

dc.subject

F-Box Proteins

dc.subject

Arabidopsis Proteins

dc.subject

Repressor Proteins

dc.subject

Crystallography, X-Ray

dc.subject

Signal Transduction

dc.subject

Binding Sites

dc.subject

Amino Acid Sequence

dc.subject

Protein Structure, Tertiary

dc.subject

Protein Binding

dc.subject

Models, Molecular

dc.subject

Molecular Sequence Data

dc.subject

Oxylipins

dc.title

Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.

dc.type

Journal article

duke.contributor.orcid

He, Sheng Yang|0000-0003-1308-498X

pubs.begin-page

400

pubs.end-page

405

pubs.issue

7322

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.organisational-group

Biology

pubs.organisational-group

Duke

pubs.publication-status

Published

pubs.volume

468

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.pdf
Size:
1.92 MB
Format:
Adobe Portable Document Format