Differential regulation of adhesion and phagocytosis of resting and activated microglia by dopamine

Loading...
Thumbnail Image

Date

2018-09-11

Journal Title

Journal ISSN

Volume Title

Repository Usage Stats

57
views
92
downloads

Citation Stats

Attention Stats

Abstract

© 2018 Fan, Chen, Pathak, Carneiro and Chung. Microglia, the immune competent cells of the central nervous system (CNS), normally exist in a resting state characterized by a ramified morphology with many processes, and become activated to amoeboid morphology in response to brain injury, infection, and a variety of neuroinflammatory stimuli. Many studies focused on how neurotransmitters affect microglia activation in pathophysiological circumstances. In this study, we tried to gain mechanistic insights on how dopamine (DA) released from neurons modulates cellular functions of resting and activated microglia. DA induced the reduction of the number of cellular processes, the increase of cell adhesion/spreading, and the increase of vimentin filaments in resting primary and BV2 microglia. In contrast to resting cells, DA downregulated the cell spreading and phagocytosis of microglia activated by LPS. DA also significantly downregulated ERK1/2 phosphorylation in activated microglia, but not in resting microglia. Downregulation of ERK1/2 by DA in activated microglia required receptor signaling. In contrast, we found a significant increase of p38MAPK activity by DA treatment in resting, but not in activated microglia. These latter effects required the uptake of DA through the high-affinity transporter but did not require receptor signaling. Activation of p38MAPK resulted in the increase of focal adhesion number via phosphorylation of paxillin at Ser83. These results indicate that DA might have a differential, depending upon the activation stage of microglia, impact on cellular functions such as adhesion and phagocytosis.

Department

Description

Provenance

Subjects

Citation

Published Version (Please cite this version)

10.3389/fncel.2018.00309

Publication Info

Fan, Yang, Zhilu Chen, Janak L Pathak, Ana MD Carneiro and Chang Y Chung (2018). Differential regulation of adhesion and phagocytosis of resting and activated microglia by dopamine. Frontiers in Cellular Neuroscience, 12. 10.3389/fncel.2018.00309 Retrieved from https://hdl.handle.net/10161/21064.

This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.


Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.