Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

dc.contributor.author

Schrott, Rose

dc.contributor.author

Murphy, Susan K

dc.contributor.author

Modliszewski, Jennifer L

dc.contributor.author

King, Dillon E

dc.contributor.author

Hill, Bendu

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Itchon-Ramos, Nilda

dc.contributor.author

Raburn, Douglas

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Price, Thomas

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Levin, Edward D

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Vandrey, Ryan

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Corcoran, David L

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Kollins, Scott H

dc.contributor.author

Mitchell, John T

dc.date.accessioned

2023-07-01T14:03:19Z

dc.date.available

2023-07-01T14:03:19Z

dc.date.issued

2021-01

dc.date.updated

2023-07-01T14:03:17Z

dc.description.abstract

Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one spermatogenic cycle). Users and controls did not significantly differ by demographics or semen analyses. Whole-genome bisulfite sequencing identified 163 CpG sites with significantly different DNA methylation in sperm between groups (P < 2.94 × 10-9). Genes associated with altered CpG sites were enriched with those involved in development, including cardiogenesis and neurodevelopment. Many of the differences in sperm DNA methylation between groups were diminished after cannabis abstinence. These results indicate that sustained cannabis abstinence significantly reduces the number of sperm showing cannabis-associated alterations at genes important for early development.

dc.identifier

dvab009

dc.identifier.issn

2058-5888

dc.identifier.issn

2058-5888

dc.identifier.uri

https://hdl.handle.net/10161/28268

dc.language

eng

dc.publisher

Oxford University Press (OUP)

dc.relation.ispartof

Environmental epigenetics

dc.relation.isversionof

10.1093/eep/dvab009

dc.subject

DNA methylation

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cannabis

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development

dc.subject

epigenetics

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sperm

dc.title

Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

dc.type

Journal article

duke.contributor.orcid

Murphy, Susan K|0000-0001-8298-7272

duke.contributor.orcid

Raburn, Douglas|0000-0002-7984-1245

duke.contributor.orcid

Price, Thomas|0000-0003-1888-306X

duke.contributor.orcid

Levin, Edward D|0000-0001-7292-8084|0000-0002-5060-9602

duke.contributor.orcid

Kollins, Scott H|0000-0001-6847-6935

duke.contributor.orcid

Mitchell, John T|0000-0002-9586-3823

pubs.begin-page

dvab009

pubs.issue

1

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Duke

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Nicholas School of the Environment

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School of Medicine

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Trinity College of Arts & Sciences

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Student

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Nicholas

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Pharmacology & Cancer Biology

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Pathology

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Psychiatry & Behavioral Sciences

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Obstetrics and Gynecology, Reproductive Endocrinology & Fertility

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Duke Cancer Institute

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Psychology & Neuroscience

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Duke Clinical Research Institute

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Environmental Sciences and Policy

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Duke Institute for Brain Sciences

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Duke Science & Society

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Psychiatry & Behavioral Sciences, Adult Psychiatry & Psychology

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Published

pubs.volume

7

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