Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.

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1981-05-15

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Abstract

The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.

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Carbon Radioisotopes, Cell Line, Fibroblasts, Glucosamine, Hexosamines, Humans, Huntington Disease, Nucleotides, Phosphorus Radioisotopes, Skin, Uridine Diphosphate N-Acetylglucosamine, Uridine Diphosphate Sugars

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https://hdl.handle.net/10161/13628

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