Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.
| dc.contributor.author | Hung, WY | |
| dc.contributor.author | Tourian, A | |
| dc.coverage.spatial | England | |
| dc.date.accessioned | 2017-02-16T17:57:22Z | |
| dc.date.available | 2017-02-16T17:57:22Z | |
| dc.date.issued | 1981-05-15 | |
| dc.description.abstract | The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts. | |
| dc.identifier | ||
| dc.identifier.issn | 0264-6021 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Portland Press Ltd. | |
| dc.relation.ispartof | Biochem J | |
| dc.subject | Carbon Radioisotopes | |
| dc.subject | Cell Line | |
| dc.subject | Fibroblasts | |
| dc.subject | Glucosamine | |
| dc.subject | Hexosamines | |
| dc.subject | Humans | |
| dc.subject | Huntington Disease | |
| dc.subject | Nucleotides | |
| dc.subject | Phosphorus Radioisotopes | |
| dc.subject | Skin | |
| dc.subject | Uridine Diphosphate N-Acetylglucosamine | |
| dc.subject | Uridine Diphosphate Sugars | |
| dc.title | Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts. | |
| dc.type | Journal article | |
| pubs.author-url | ||
| pubs.begin-page | 495 | |
| pubs.end-page | 498 | |
| pubs.issue | 2 | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Neurology | |
| pubs.organisational-group | School of Medicine | |
| pubs.publication-status | Published | |
| pubs.volume | 196 |
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