Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium.

dc.contributor.author

Abar, Bijan

dc.contributor.author

Kelly, Cambre

dc.contributor.author

Pham, Anh

dc.contributor.author

Allen, Nicholas

dc.contributor.author

Barber, Helena

dc.contributor.author

Kelly, Alexander

dc.contributor.author

Mirando, Anthony J

dc.contributor.author

Hilton, Matthew J

dc.contributor.author

Gall, Ken

dc.contributor.author

Adams, Samuel B

dc.date.accessioned

2022-09-01T18:06:56Z

dc.date.available

2022-09-01T18:06:56Z

dc.date.issued

2021-10

dc.date.updated

2022-09-01T18:06:55Z

dc.description.abstract

Critical-sized defects remain a significant challenge in orthopaedics. 3D printed scaffolds are a promising treatment but are still limited due to inconsistent osseous integration. The goal of the study is to understand how changing the surface roughness of 3D printed titanium either by surface treatment or artificially printing rough topography impacts the mechanical and biological properties of 3D printed titanium. Titanium tensile samples and discs were printed via laser powder bed fusion. Roughness was manipulated by post-processing printed samples or by directly printing rough features. Experimental groups in order of increasing surface roughness were Polished, Blasted, As Built, Sprouts, and Rough Sprouts. Tensile behavior of samples showed reduced strength with increasing surface roughness. MC3T3 pre-osteoblasts were seeded on discs and analyzed for cellular proliferation, differentiation, and matrix deposition at 0, 2, and 4 weeks. Printing roughness diminished mechanical properties such as tensile strength and ductility without clear benefit to cell growth. Roughness features were printed on mesoscale, unlike samples in literature in which roughness on microscale demonstrated an increase in cell activity. The data suggest that printing artificial roughness on titanium scaffold is not an effective strategy to promote osseous integration.

dc.identifier.issn

1549-3296

dc.identifier.issn

1552-4965

dc.identifier.uri

https://hdl.handle.net/10161/25648

dc.language

eng

dc.publisher

Wiley

dc.relation.ispartof

Journal of biomedical materials research. Part A

dc.relation.isversionof

10.1002/jbm.a.37172

dc.subject

Cell Line

dc.subject

Osteoblasts

dc.subject

Animals

dc.subject

Mice

dc.subject

Titanium

dc.subject

Alloys

dc.subject

Collagen

dc.subject

Adaptor Proteins, Signal Transducing

dc.subject

Osteocalcin

dc.subject

Surface Properties

dc.subject

Stress, Mechanical

dc.subject

Tensile Strength

dc.subject

Printing, Three-Dimensional

dc.title

Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium.

dc.type

Journal article

duke.contributor.orcid

Allen, Nicholas|0000-0002-4866-125X

duke.contributor.orcid

Hilton, Matthew J|0000-0003-3165-267X

duke.contributor.orcid

Adams, Samuel B|0000-0003-1020-1167

pubs.begin-page

1792

pubs.end-page

1802

pubs.issue

10

pubs.organisational-group

Duke

pubs.organisational-group

Pratt School of Engineering

pubs.organisational-group

School of Medicine

pubs.organisational-group

Student

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Cell Biology

pubs.organisational-group

Biomedical Engineering

pubs.organisational-group

Thomas Lord Department of Mechanical Engineering and Materials Science

pubs.organisational-group

Orthopaedic Surgery

pubs.organisational-group

Pathology

pubs.organisational-group

Regeneration Next Initiative

pubs.publication-status

Published

pubs.volume

109

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Effect of Surface Topography on in vitro Osteoblast Function.pdf
Size:
2.98 MB
Format:
Adobe Portable Document Format