Engineering of obligate intracellular bacteria: progress, challenges and paradigms.

dc.contributor.author

McClure, Erin E

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Chávez, Adela S Oliva

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Shaw, Dana K

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Carlyon, Jason A

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Ganta, Roman R

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Noh, Susan M

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Wood, David O

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Bavoil, Patrik M

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Brayton, Kelly A

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Martinez, Juan J

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McBride, Jere W

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Valdivia, Raphael H

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Munderloh, Ulrike G

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Pedra, Joao HF

dc.coverage.spatial

England

dc.date.accessioned

2017-08-01T15:29:20Z

dc.date.available

2017-08-01T15:29:20Z

dc.date.issued

2017-06-19

dc.description.abstract

It is estimated that approximately one billion people are at risk of infection with obligate intracellular bacteria, but little is known about the underlying mechanisms that govern their life cycles. The difficulty in studying Chlamydia spp., Coxiella spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp. and Orientia spp. is, in part, due to their genetic intractability. Recently, genetic tools have been developed; however, optimizing the genomic manipulation of obligate intracellular bacteria remains challenging. In this Review, we describe the progress in, as well as the constraints that hinder, the systematic development of a genetic toolbox for obligate intracellular bacteria. We highlight how the use of genetically manipulated pathogens has facilitated a better understanding of microbial pathogenesis and immunity, and how the engineering of obligate intracellular bacteria could enable the discovery of novel signalling circuits in host-pathogen interactions.

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/28626230

dc.identifier

nrmicro.2017.59

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1740-1534

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https://hdl.handle.net/10161/15149

dc.language

eng

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Springer Science and Business Media LLC

dc.relation.ispartof

Nat Rev Microbiol

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10.1038/nrmicro.2017.59

dc.title

Engineering of obligate intracellular bacteria: progress, challenges and paradigms.

dc.type

Journal article

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/28626230

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Duke

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Molecular Genetics and Microbiology

pubs.organisational-group

School of Medicine

pubs.publication-status

Published online

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