Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring.

dc.contributor.author

Vidal, AC

dc.contributor.author

Murphy, SK

dc.contributor.author

Murtha, AP

dc.contributor.author

Schildkraut, JM

dc.contributor.author

Soubry, A

dc.contributor.author

Huang, Z

dc.contributor.author

Neelon, SEB

dc.contributor.author

Fuemmeler, B

dc.contributor.author

Iversen, E

dc.contributor.author

Wang, F

dc.contributor.author

Kurtzberg, J

dc.contributor.author

Jirtle, RL

dc.contributor.author

Hoyo, C

dc.date.accessioned

2022-03-23T20:26:27Z

dc.date.available

2022-03-23T20:26:27Z

dc.date.issued

2013-07

dc.date.updated

2022-03-23T20:26:26Z

dc.description.abstract

Objectives

Low birth weight (LBW) has been associated with common adult-onset chronic diseases, including obesity, cardiovascular disease, type II diabetes and some cancers. The etiology of LBW is multi-factorial. However, recent evidence suggests exposure to antibiotics may also increase the risk of LBW. The mechanisms underlying this association are unknown, although epigenetic mechanisms are hypothesized. In this study, we evaluated the association between maternal antibiotic use and LBW and examined the potential role of altered DNA methylation that controls growth regulatory imprinted genes in these associations.

Methods

Between 2009-2011, 397 pregnant women were enrolled and followed until delivery. Prenatal antibiotic use was ascertained through maternal self-report. Imprinted genes methylation levels were measured at differentially methylated regions (DMRs) using bisulfite pyrosequencing. Generalized linear models were used to examine associations among antibiotic use, birth weight and DMR methylation fractions.

Results

After adjusting for infant gender, race/ethnicity, maternal body mass index, delivery route, gestational weight gain, gestational age at delivery, folic acid intake, physical activity, maternal smoking and parity, antibiotic use during pregnancy was associated with 138 g lower birth weight compared with non-antibiotic use (β-coefficient=-132.99, s.e.=50.70, P=0.008). These associations were strongest in newborns of women who reported antibiotic use other than penicillins (β-coefficient=-135.57, s.e.=57.38, P=0.02). Methylation at five DMRs, IGF2 (P=0.05), H19 (P=0.15), PLAGL1 (P=0.01), MEG3 (P=0.006) and PEG3 (P=0.08), was associated with maternal antibiotic use; among these, only methylation at the PLAGL1 DMR was also associated with birth weight.

Conclusion

We report an inverse association between in utero exposure to antibiotics and lower infant birth weight and provide the first empirical evidence supporting imprinted gene plasticity in these associations.
dc.identifier

ijo201347

dc.identifier.issn

0307-0565

dc.identifier.issn

1476-5497

dc.identifier.uri

https://hdl.handle.net/10161/24687

dc.language

eng

dc.publisher

Springer Science and Business Media LLC

dc.relation.ispartof

International journal of obesity (2005)

dc.relation.isversionof

10.1038/ijo.2013.47

dc.subject

Humans

dc.subject

Neoplasms

dc.subject

Prenatal Exposure Delayed Effects

dc.subject

Cardiovascular Diseases

dc.subject

Obesity

dc.subject

Birth Weight

dc.subject

Intercellular Signaling Peptides and Proteins

dc.subject

Insulin-Like Growth Factor II

dc.subject

Proteins

dc.subject

Calcium-Binding Proteins

dc.subject

Cell Cycle Proteins

dc.subject

Sarcoglycans

dc.subject

Membrane Proteins

dc.subject

Tumor Suppressor Proteins

dc.subject

Nerve Tissue Proteins

dc.subject

Transcription Factors

dc.subject

Anti-Bacterial Agents

dc.subject

Prospective Studies

dc.subject

Sequence Analysis, DNA

dc.subject

DNA Methylation

dc.subject

Epigenesis, Genetic

dc.subject

Genomic Imprinting

dc.subject

Fetal Development

dc.subject

Pregnancy

dc.subject

Adult

dc.subject

Infant, Newborn

dc.subject

Infant, Low Birth Weight

dc.subject

United States

dc.subject

Female

dc.subject

Kruppel-Like Transcription Factors

dc.subject

RNA, Long Noncoding

dc.title

Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring.

dc.type

Journal article

duke.contributor.orcid

Murphy, SK|0000-0001-8298-7272

duke.contributor.orcid

Kurtzberg, J|0000-0002-3370-0703

pubs.begin-page

907

pubs.end-page

913

pubs.issue

7

pubs.organisational-group

Duke

pubs.organisational-group

Nicholas School of the Environment

pubs.organisational-group

Sanford School of Public Policy

pubs.organisational-group

School of Medicine

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.organisational-group

Faculty

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

Family Medicine and Community Health

pubs.organisational-group

Pathology

pubs.organisational-group

Pediatrics

pubs.organisational-group

Family Medicine and Community Health, Prevention Research

pubs.organisational-group

Pediatrics, Primary Care Pediatrics

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Statistical Science

pubs.organisational-group

Environmental Sciences and Policy

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.organisational-group

Initiatives

pubs.organisational-group

Center for Child and Family Policy

pubs.organisational-group

Duke Innovation & Entrepreneurship

pubs.organisational-group

Pediatrics, Transplant and Cellular Therapy

pubs.publication-status

Published

pubs.volume

37

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
p264 Vidal.pdf
Size:
328.7 KB
Format:
Adobe Portable Document Format
Description:
Published version