An <i>In Vitro</i> Microfluidic Alveolus Model to Study Lung Biomechanics.
| dc.contributor.author | Kumar, Vardhman | |
| dc.contributor.author | Madhurakkat Perikamana, Sajeesh Kumar | |
| dc.contributor.author | Tata, Aleksandra | |
| dc.contributor.author | Hoque, Jiaul | |
| dc.contributor.author | Gilpin, Anna | |
| dc.contributor.author | Tata, Purushothama Rao | |
| dc.contributor.author | Varghese, Shyni | |
| dc.date.accessioned | 2023-10-01T13:41:18Z | |
| dc.date.available | 2023-10-01T13:41:18Z | |
| dc.date.issued | 2022-01 | |
| dc.date.updated | 2023-10-01T13:41:16Z | |
| dc.description.abstract | The gas exchange units of the lung, the alveoli, are mechanically active and undergo cyclic deformation during breathing. The epithelial cells that line the alveoli contribute to lung function by reducing surface tension via surfactant secretion, which is highly influenced by the breathing-associated mechanical cues. These spatially heterogeneous mechanical cues have been linked to several physiological and pathophysiological states. Here, we describe the development of a microfluidically assisted lung cell culture model that incorporates heterogeneous cyclic stretching to mimic alveolar respiratory motions. Employing this device, we have examined the effects of respiratory biomechanics (associated with breathing-like movements) and strain heterogeneity on alveolar epithelial cell functions. Furthermore, we have assessed the potential application of this platform to model altered matrix compliance associated with lung pathogenesis and ventilator-induced lung injury. Lung microphysiological platforms incorporating human cells and dynamic biomechanics could serve as an important tool to delineate the role of alveolar micromechanics in physiological and pathological outcomes in the lung. | |
| dc.identifier | 848699 | |
| dc.identifier.issn | 2296-4185 | |
| dc.identifier.issn | 2296-4185 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Frontiers Media SA | |
| dc.relation.ispartof | Frontiers in bioengineering and biotechnology | |
| dc.relation.isversionof | 10.3389/fbioe.2022.848699 | |
| dc.subject | in vitro system | |
| dc.subject | lung | |
| dc.subject | microfluidics | |
| dc.subject | microphysiological system | |
| dc.subject | organ-on-a-chip | |
| dc.title | An In Vitro Microfluidic Alveolus Model to Study Lung Biomechanics. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Tata, Aleksandra|0000-0003-3270-0485 | |
| duke.contributor.orcid | Tata, Purushothama Rao|0000-0003-4837-0337 | |
| duke.contributor.orcid | Varghese, Shyni|0000-0002-0004-7947 | |
| pubs.begin-page | 848699 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Student | |
| pubs.organisational-group | Pratt | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Orthopaedic Surgery | |
| pubs.organisational-group | Medicine, Pulmonary, Allergy, and Critical Care Medicine | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Regeneration Next Initiative | |
| pubs.publication-status | Published | |
| pubs.volume | 10 |
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