Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression.

dc.contributor.author

Yanai, Itai

dc.contributor.author

Baugh, L Ryan

dc.contributor.author

Smith, Jessica J

dc.contributor.author

Roehrig, Casey

dc.contributor.author

Shen-Orr, Shai S

dc.contributor.author

Claggett, Julia M

dc.contributor.author

Hill, Andrew A

dc.contributor.author

Slonim, Donna K

dc.contributor.author

Hunter, Craig P

dc.coverage.spatial

England

dc.date.accessioned

2017-03-01T15:33:17Z

dc.date.available

2017-03-01T15:33:17Z

dc.date.issued

2008

dc.description.abstract

Biological networks are inherently modular, yet little is known about how modules are assembled to enable coordinated and complex functions. We used RNAi and time series, whole-genome microarray analyses to systematically perturb and characterize components of a Caenorhabditis elegans lineage-specific transcriptional regulatory network. These data are supported by selected reporter gene analyses and comprehensive yeast one-hybrid and promoter sequence analyses. Based on these results, we define and characterize two modules composed of muscle- and epidermal-specifying transcription factors that function together within a single cell lineage to robustly specify multiple cell types. The expression of these two modules, although positively regulated by a common factor, is reliably segregated among daughter cells. Our analyses indicate that these modules repress each other, and we propose that this cross-inhibition coupled with their relative time of induction function to enhance the initial asymmetry in their expression patterns, thus leading to the observed invariant gene expression patterns and cell lineage. The coupling of asynchronous and topologically distinct modules may be a general principle of module assembly that functions to potentiate genetic switches.

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/18277379

dc.identifier

msb20086

dc.identifier.eissn

1744-4292

dc.identifier.uri

https://hdl.handle.net/10161/13730

dc.language

eng

dc.publisher

EMBO

dc.relation.ispartof

Mol Syst Biol

dc.relation.isversionof

10.1038/msb.2008.6

dc.subject

Animals

dc.subject

Body Patterning

dc.subject

Caenorhabditis elegans

dc.subject

Caenorhabditis elegans Proteins

dc.subject

Cell Lineage

dc.subject

Embryo, Nonmammalian

dc.subject

Epidermis

dc.subject

Female

dc.subject

Gene Expression Regulation, Developmental

dc.subject

Gene Regulatory Networks

dc.subject

Genes, Helminth

dc.subject

Genes, Regulator

dc.subject

Genes, Reporter

dc.subject

Models, Biological

dc.subject

Muscles

dc.subject

Mutation

dc.subject

RNA Interference

dc.subject

RNA-Binding Proteins

dc.subject

Sensitivity and Specificity

dc.subject

Transcription, Genetic

dc.title

Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression.

dc.type

Journal article

duke.contributor.orcid

Baugh, L Ryan|0000-0003-2148-5492

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/18277379

pubs.begin-page

163

pubs.organisational-group

Biology

pubs.organisational-group

Duke

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.publication-status

Published

pubs.volume

4

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression.pdf
Size:
343.64 KB
Format:
Adobe Portable Document Format