Quantitative and qualitative preparations of bacterial outer membrane vesicles
| dc.contributor.author | Chutkan, H | |
| dc.contributor.author | MacDonald, IA | |
| dc.contributor.author | Manning, AJ | |
| dc.contributor.author | Kuehn, MJ | |
| dc.date.accessioned | 2023-01-03T18:44:35Z | |
| dc.date.available | 2023-01-03T18:44:35Z | |
| dc.date.issued | 2013-12-01 | |
| dc.date.updated | 2023-01-03T18:44:35Z | |
| dc.description.abstract | Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains. © Springer Science+Business Media New York 2013. | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.uri | ||
| dc.relation.ispartof | Methods in Molecular Biology | |
| dc.relation.isversionof | 10.1007/978-1-62703-245-2-16 | |
| dc.title | Quantitative and qualitative preparations of bacterial outer membrane vesicles | |
| dc.type | Journal article | |
| pubs.begin-page | 259 | |
| pubs.end-page | 272 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Biochemistry | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Molecular Genetics and Microbiology | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.volume | 966 |
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