High-speed widefield photoacoustic microscopy of small-animal hemodynamics.
| dc.contributor.author | Lan, Bangxin | |
| dc.contributor.author | Liu, Wei | |
| dc.contributor.author | Wang, Ya-Chao | |
| dc.contributor.author | Shi, Junhui | |
| dc.contributor.author | Li, Yang | |
| dc.contributor.author | Xu, Song | |
| dc.contributor.author | Sheng, Huaxin | |
| dc.contributor.author | Zhou, Qifa | |
| dc.contributor.author | Zou, Jun | |
| dc.contributor.author | Hoffmann, Ulrike | |
| dc.contributor.author | Yang, Wei | |
| dc.contributor.author | Yao, Junjie | |
| dc.date.accessioned | 2021-06-01T13:46:48Z | |
| dc.date.available | 2021-06-01T13:46:48Z | |
| dc.date.issued | 2018-10 | |
| dc.date.updated | 2021-06-01T13:46:48Z | |
| dc.description.abstract | Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest. | |
| dc.identifier | 340439 | |
| dc.identifier.issn | 2156-7085 | |
| dc.identifier.issn | 2156-7085 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Optica Publishing Group | |
| dc.relation.ispartof | Biomedical optics express | |
| dc.relation.isversionof | 10.1364/boe.9.004689 | |
| dc.subject | Science & Technology | |
| dc.subject | Life Sciences & Biomedicine | |
| dc.subject | Physical Sciences | |
| dc.subject | Biochemical Research Methods | |
| dc.subject | Optics | |
| dc.subject | Radiology, Nuclear Medicine & Medical Imaging | |
| dc.subject | Biochemistry & Molecular Biology | |
| dc.subject | OPTOACOUSTIC TOMOGRAPHY MSOT | |
| dc.subject | MOUSE-BRAIN | |
| dc.subject | RESOLUTION | |
| dc.subject | EPINEPHRINE | |
| dc.subject | SCANNER | |
| dc.subject | STROKE | |
| dc.subject | VASCULATURE | |
| dc.subject | SENSITIVITY | |
| dc.subject | LASER | |
| dc.title | High-speed widefield photoacoustic microscopy of small-animal hemodynamics. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Sheng, Huaxin|0000-0002-4325-2940 | |
| duke.contributor.orcid | Yang, Wei|0000-0001-5719-4393 | |
| duke.contributor.orcid | Yao, Junjie|0000-0002-2381-706X | |
| pubs.begin-page | 4689 | |
| pubs.end-page | 4701 | |
| pubs.issue | 10 | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Anesthesiology | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Anesthesiology, Neuroanesthesia | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.publication-status | Published | |
| pubs.volume | 9 |