H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza vaccination.

dc.contributor.author

Moody, M Anthony

dc.contributor.author

Zhang, Ruijun

dc.contributor.author

Walter, Emmanuel B

dc.contributor.author

Woods, Christopher W

dc.contributor.author

Ginsburg, Geoffrey S

dc.contributor.author

McClain, Micah T

dc.contributor.author

Denny, Thomas N

dc.contributor.author

Chen, Xi

dc.contributor.author

Munshaw, Supriya

dc.contributor.author

Marshall, Dawn J

dc.contributor.author

Whitesides, John F

dc.contributor.author

Drinker, Mark S

dc.contributor.author

Amos, Joshua D

dc.contributor.author

Gurley, Thaddeus C

dc.contributor.author

Eudailey, Joshua A

dc.contributor.author

Foulger, Andrew

dc.contributor.author

DeRosa, Katherine R

dc.contributor.author

Parks, Robert

dc.contributor.author

Meyerhoff, R Ryan

dc.contributor.author

Yu, Jae-Sung

dc.contributor.author

Kozink, Daniel M

dc.contributor.author

Barefoot, Brice E

dc.contributor.author

Ramsburg, Elizabeth A

dc.contributor.author

Khurana, Surender

dc.contributor.author

Golding, Hana

dc.contributor.author

Vandergrift, Nathan A

dc.contributor.author

Alam, S Munir

dc.contributor.author

Tomaras, Georgia D

dc.contributor.author

Kepler, Thomas B

dc.contributor.author

Kelsoe, Garnett

dc.contributor.author

Liao, Hua-Xin

dc.contributor.author

Haynes, Barton F

dc.contributor.editor

Randall, Troy D

dc.date.accessioned

2021-01-04T22:22:35Z

dc.date.available

2021-01-04T22:22:35Z

dc.date.issued

2011-01

dc.date.updated

2021-01-04T22:22:33Z

dc.description.abstract

During the recent H1N1 influenza pandemic, excess morbidity and mortality was seen in young but not older adults suggesting that prior infection with influenza strains may have protected older subjects. In contrast, a history of recent seasonal trivalent vaccine in younger adults was not associated with protection.To study hemagglutinin (HA) antibody responses in influenza immunization and infection, we have studied the day 7 plasma cell repertoires of subjects immunized with seasonal trivalent inactivated influenza vaccine (TIV) and compared them to the plasma cell repertoires of subjects experimentally infected (EI) with influenza H3N2 A/Wisconsin/67/2005. The majority of circulating plasma cells after TIV produced influenza-specific antibodies, while most plasma cells after EI produced antibodies that did not react with influenza HA. While anti-HA antibodies from TIV subjects were primarily reactive with single or few HA strains, anti-HA antibodies from EI subjects were isolated that reacted with multiple HA strains. Plasma cell-derived anti-HA antibodies from TIV subjects showed more evidence of clonal expansion compared with antibodies from EI subjects. From an H3N2-infected subject, we isolated a 4-member clonal lineage of broadly cross-reactive antibodies that bound to multiple HA subtypes and neutralized both H1N1 and H3N2 viruses. This broad reactivity was not detected in post-infection plasma suggesting this broadly reactive clonal lineage was not immunodominant in this subject.The presence of broadly reactive subdominant antibody responses in some EI subjects suggests that improved vaccine designs that make broadly reactive antibody responses immunodominant could protect against novel influenza strains.

dc.identifier

PONE-D-11-08028

dc.identifier.issn

1932-6203

dc.identifier.issn

1932-6203

dc.identifier.uri

https://hdl.handle.net/10161/22006

dc.language

eng

dc.publisher

Public Library of Science (PLoS)

dc.relation.ispartof

PloS one

dc.relation.isversionof

10.1371/journal.pone.0025797

dc.subject

Humans

dc.subject

Hemagglutinin Glycoproteins, Influenza Virus

dc.subject

Influenza Vaccines

dc.subject

Antibodies, Viral

dc.subject

Fluorescent Antibody Technique, Indirect

dc.subject

Enzyme-Linked Immunosorbent Assay

dc.subject

Surface Plasmon Resonance

dc.subject

Reverse Transcriptase Polymerase Chain Reaction

dc.subject

Antibody Specificity

dc.subject

Cross Reactions

dc.subject

Influenza, Human

dc.subject

Influenza A Virus, H3N2 Subtype

dc.title

H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza vaccination.

dc.type

Journal article

duke.contributor.orcid

Moody, M Anthony|0000-0002-3890-5855

duke.contributor.orcid

Woods, Christopher W|0000-0001-7240-2453

duke.contributor.orcid

Ginsburg, Geoffrey S|0000-0003-4739-9808

duke.contributor.orcid

Alam, S Munir|0000-0003-0941-0703

duke.contributor.orcid

Tomaras, Georgia D|0000-0001-8076-1931

duke.contributor.orcid

Kelsoe, Garnett|0000-0002-8770-040X

pubs.begin-page

e25797

pubs.issue

10

pubs.organisational-group

School of Medicine

pubs.organisational-group

Duke Human Vaccine Institute

pubs.organisational-group

Duke Global Health Institute

pubs.organisational-group

Medicine, Duke Human Vaccine Institute

pubs.organisational-group

Duke

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

University Institutes and Centers

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.organisational-group

Medicine

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Immunology

pubs.organisational-group

Surgery, Surgical Sciences

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Surgery

pubs.organisational-group

Pediatrics, Infectious Diseases

pubs.organisational-group

Pediatrics

pubs.organisational-group

Pathology

pubs.organisational-group

Nursing

pubs.organisational-group

Medicine, Cardiology

pubs.organisational-group

School of Nursing

pubs.organisational-group

Medicine, Infectious Diseases

pubs.organisational-group

Faculty

pubs.organisational-group

Molecular Genetics and Microbiology

pubs.organisational-group

Duke Innovation & Entrepreneurship

pubs.organisational-group

Initiatives

pubs.organisational-group

Duke Clinical Research Institute

pubs.organisational-group

Pediatrics, Primary Care Pediatrics

pubs.organisational-group

Staff

pubs.publication-status

Published

pubs.volume

6

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza.pdf
Size:
638.37 KB
Format:
Adobe Portable Document Format