RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin.

dc.contributor.author

Johnson, WL

dc.contributor.author

Yewdell, WT

dc.contributor.author

Bell, JC

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McNulty, SM

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Duda, Z

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O'Neill, RJ

dc.contributor.author

Sullivan, BA

dc.contributor.author

Straight, AF

dc.coverage.spatial

England

dc.date.accessioned

2017-08-21T22:43:07Z

dc.date.available

2017-08-21T22:43:07Z

dc.date.issued

2017-08-01

dc.description.abstract

Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear. Here, we demonstrate that chromatin-associated RNA contributes to the stable association of SUV39H1 with constitutive heterochromatin in human cells. We find that RNA associated with mitotic chromosomes is concentrated at pericentric heterochromatin, and is encoded, in part, by repetitive α-satellite sequences, which are retained in cis at their transcription sites. Purified SUV39H1 directly binds nucleic acids through its chromodomain; and in cells, SUV39H1 associates with α-satellite RNA transcripts. Furthermore, nucleic acid binding mutants destabilize the association of SUV39H1 with chromatin in mitotic and interphase cells - effects that can be recapitulated by RNase treatment or RNA polymerase inhibition - and cause defects in heterochromatin function. Collectively, our findings uncover a previously unrealized function for chromatin-associated RNA in regulating constitutive heterochromatin in human cells.

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/28760200

dc.identifier.eissn

2050-084X

dc.identifier.uri

https://hdl.handle.net/10161/15324

dc.publisher

eLife Sciences Publications, Ltd

dc.relation.ispartof

Elife

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10.7554/eLife.25299

dc.subject

SUV39H1

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cell biology

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chromatin

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chromosomes

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genes

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heterochromatin

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histone methylation

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human

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noncoding RNA

dc.title

RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin.

dc.type

Conference

duke.contributor.orcid

Sullivan, BA|0000-0001-5216-4603

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/28760200

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Duke

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

Molecular Genetics and Microbiology

pubs.organisational-group

School of Medicine

pubs.publication-status

Published online

pubs.volume

6

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