Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.

Abstract

Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C+ ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C+ Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes.

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Citation

Published Version (Please cite this version)

10.1002/anie.201902693

Publication Info

Stelling, Allison L, Amy Y Liu, Wenjie Zeng, Raul Salinas, Maria A Schumacher and Hashim M Al-Hashimi (2019). Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions. Angewandte Chemie (International ed. in English), 58(35). pp. 12010–12013. 10.1002/anie.201902693 Retrieved from https://hdl.handle.net/10161/24729.

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Scholars@Duke

Schumacher

Maria Anne Schumacher

Nanaline H. Duke Distinguished Professor of Biochemistry
Al-Hashimi

Hashim Al-Hashimi

Adjunct Professor in the Department of Biochemistry

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