Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.
dc.contributor.author | Stelling, Allison L | |
dc.contributor.author | Liu, Amy Y | |
dc.contributor.author | Zeng, Wenjie | |
dc.contributor.author | Salinas, Raul | |
dc.contributor.author | Schumacher, Maria A | |
dc.contributor.author | Al-Hashimi, Hashim M | |
dc.date.accessioned | 2022-03-28T19:10:35Z | |
dc.date.available | 2022-03-28T19:10:35Z | |
dc.date.issued | 2019-08 | |
dc.date.updated | 2022-03-28T19:10:34Z | |
dc.description.abstract | Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C+ ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C+ Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes. | |
dc.identifier.issn | 1433-7851 | |
dc.identifier.issn | 1521-3773 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Wiley | |
dc.relation.ispartof | Angewandte Chemie (International ed. in English) | |
dc.relation.isversionof | 10.1002/anie.201902693 | |
dc.subject | Cytosine | |
dc.subject | Guanine | |
dc.subject | TATA-Box Binding Protein | |
dc.subject | DNA | |
dc.subject | Crystallography, X-Ray | |
dc.subject | Spectrophotometry, Infrared | |
dc.subject | Nucleic Acid Conformation | |
dc.subject | Base Pairing | |
dc.title | Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions. | |
dc.type | Journal article | |
duke.contributor.orcid | Salinas, Raul|0000-0001-9011-683X | |
pubs.begin-page | 12010 | |
pubs.end-page | 12013 | |
pubs.issue | 35 | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Staff | |
pubs.organisational-group | Basic Science Departments | |
pubs.organisational-group | Biochemistry | |
pubs.publication-status | Published | |
pubs.volume | 58 |
Files
Original bundle
- Name:
- Stelling_et_al-2019-Angewandte_Chemie_International_Edition.pdf
- Size:
- 2.68 MB
- Format:
- Adobe Portable Document Format
- Description:
- Published version