Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.

dc.contributor.author

Stelling, Allison L

dc.contributor.author

Liu, Amy Y

dc.contributor.author

Zeng, Wenjie

dc.contributor.author

Salinas, Raul

dc.contributor.author

Schumacher, Maria A

dc.contributor.author

Al-Hashimi, Hashim M

dc.date.accessioned

2022-03-28T19:10:35Z

dc.date.available

2022-03-28T19:10:35Z

dc.date.issued

2019-08

dc.date.updated

2022-03-28T19:10:34Z

dc.description.abstract

Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C+ ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C+ Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes.

dc.identifier.issn

1433-7851

dc.identifier.issn

1521-3773

dc.identifier.uri

https://hdl.handle.net/10161/24729

dc.language

eng

dc.publisher

Wiley

dc.relation.ispartof

Angewandte Chemie (International ed. in English)

dc.relation.isversionof

10.1002/anie.201902693

dc.subject

Cytosine

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Guanine

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TATA-Box Binding Protein

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DNA

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Crystallography, X-Ray

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Spectrophotometry, Infrared

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Nucleic Acid Conformation

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Base Pairing

dc.title

Infrared Spectroscopic Observation of a G-C+ Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.

dc.type

Journal article

duke.contributor.orcid

Salinas, Raul|0000-0001-9011-683X

pubs.begin-page

12010

pubs.end-page

12013

pubs.issue

35

pubs.organisational-group

Duke

pubs.organisational-group

School of Medicine

pubs.organisational-group

Staff

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Biochemistry

pubs.publication-status

Published

pubs.volume

58

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