In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.
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2011-06-01
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We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.
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Matthews, Thomas E, Jesse W Wilson, Simone Degan, Mary Jane Simpson, Jane Y Jin, Jennifer Y Zhang and Warren S Warren (2011). In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Express, 2(6). pp. 1576–1583. 10.1364/BOE.2.001576 Retrieved from https://hdl.handle.net/10161/15168.
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Warren S. Warren
Our work focuses on the design and application of what might best be called novel pulsed techniques, using controlled radiation fields to alter dynamics. The heart of the work is chemical physics, and most of what we do is ultrafast laser spectroscopy or nuclear magnetic resonance. It generally involves an intimate mixture of theory and experiment: recent publications are roughly an equal mix of pencil- and-paper theory, computer calculations with our workstations, and experiments. Collaborations also play an important role, particularly for medical applications.
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