Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced.

Dal Porto, Joseph M; Haberman, Ann M; Kelsoe, Garnett; Shlomchik, Mark J

Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced.

dc.contributor.author	Dal Porto, Joseph M
dc.contributor.author	Haberman, Ann M
dc.contributor.author	Kelsoe, Garnett
dc.contributor.author	Shlomchik, Mark J
dc.coverage.spatial	United States
dc.date.accessioned	2015-11-18T16:43:53Z
dc.date.issued	2002-05-06
dc.description.abstract	To understand the relationship between the affinity of the B cell antigen receptor (BCR) and the immune response to antigen, two lines of immunoglobulin H chain transgenic (Tg) mice were created. H50Gmu(a) and T1(V23)mu(a) mice express mu H chain transgenes that associate with the lambda1 L chains to bind the (4-hydroxy-3-nitrophenyl)acetyl hapten with association constants (K(a)s) of only 1.2 x 10(5) M(-1) and 3 x 10(4) M(-1), respectively. Both lines mounted substantial antibody-forming cell (AFC) and germinal center (GC) responses. H50Gmu(a) Tg mice also generated memory B cells. T1(V23)mu(a) B cells formed AFC and GCs, but were largely replaced in late GCs by antigen-specific cells that express endogenous BCRs. Thus, B lymphocytes carrying BCRs with affinities previously thought to be irrelevant in specific immune responses are in fact capable of complete T cell-dependent immune responses when relieved of substantial competition from other B cells. The failure to observe such B cells normally in late primary responses and in memory B cell populations is the result of competition, rather than an intrinsic inability of low affinity B cells.
dc.identifier	http://www.ncbi.nlm.nih.gov/pubmed/11994427
dc.identifier.issn	0022-1007
dc.identifier.uri	https://hdl.handle.net/10161/10908
dc.language	eng
dc.publisher	Rockefeller University Press
dc.relation.ispartof	J Exp Med
dc.subject	Animals
dc.subject	Antibody Formation
dc.subject	B-Lymphocytes
dc.subject	Gene Rearrangement, B-Lymphocyte
dc.subject	Immunoglobulin Heavy Chains
dc.subject	Immunologic Memory
dc.subject	Mice
dc.subject	Mice, SCID
dc.subject	Mice, Transgenic
dc.subject	Receptors, Antigen, B-Cell
dc.subject	T-Lymphocytes
dc.title	Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced.
dc.type	Journal article
pubs.author-url	http://www.ncbi.nlm.nih.gov/pubmed/11994427
pubs.begin-page	1215
pubs.end-page	1221
pubs.issue	9
pubs.organisational-group	Basic Science Departments
pubs.organisational-group	Duke
pubs.organisational-group	Duke Cancer Institute
pubs.organisational-group	Duke Human Vaccine Institute
pubs.organisational-group	Immunology
pubs.organisational-group	Institutes and Centers
pubs.organisational-group	School of Medicine
pubs.publication-status	Published
pubs.volume	195

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