Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
| dc.contributor.author | Jung, Y | |
| dc.contributor.author | Ji, H | |
| dc.contributor.author | Chen, Z | |
| dc.contributor.author | Fai Chan, H | |
| dc.contributor.author | Atchison, L | |
| dc.contributor.author | Klitzman, B | |
| dc.contributor.author | Truskey, G | |
| dc.contributor.author | Leong, KW | |
| dc.coverage.spatial | England | |
| dc.date.accessioned | 2016-06-20T22:05:21Z | |
| dc.date.issued | 2015-10-12 | |
| dc.description.abstract | Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. Hypothesizing that a scaffold-free TEBV may be advantageous, we constructed a tubular structure (1 mm i.d.) from aligned human mesenchymal cell sheets (hMSC) as the wall and human endothelial progenitor cell (hEPC) coating as the lumen. The burst pressure of the scaffold-free TEBV was above 200 mmHg after three weeks of sequential culture in a rotating wall bioreactor and perfusion at 6.8 dynes/cm(2). The interwoven organization of the cell layers and extensive extracellular matrix (ECM) formation of the hMSC-based TEBV resembled that of native blood vessels. The TEBV exhibited flow-mediated vasodilation, vasoconstriction after exposure to 1 μM phenylephrine and released nitric oxide in a manner similar to that of porcine femoral vein. HL-60 cells attached to the TEBV lumen after TNF-α activation to suggest a functional endothelium. This study demonstrates the potential of a hEPC endothelialized hMSC-based TEBV for drug screening. | |
| dc.identifier | ||
| dc.identifier | srep15116 | |
| dc.identifier.eissn | 2045-2322 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.relation.ispartof | Sci Rep | |
| dc.relation.isversionof | 10.1038/srep15116 | |
| dc.subject | Animals | |
| dc.subject | Bioreactors | |
| dc.subject | Blood Vessel Prosthesis | |
| dc.subject | Cell Adhesion | |
| dc.subject | Cell Culture Techniques | |
| dc.subject | Extracellular Matrix | |
| dc.subject | Femoral Vein | |
| dc.subject | HL-60 Cells | |
| dc.subject | Human Umbilical Vein Endothelial Cells | |
| dc.subject | Humans | |
| dc.subject | Mesenchymal Stromal Cells | |
| dc.subject | Nitric Oxide | |
| dc.subject | Perfusion | |
| dc.subject | Phenylephrine | |
| dc.subject | Pressure | |
| dc.subject | Swine | |
| dc.subject | Tissue Engineering | |
| dc.subject | Tumor Necrosis Factor-alpha | |
| dc.subject | Vasoconstriction | |
| dc.subject | Vasodilation | |
| dc.title | Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Truskey, G|0000-0002-6885-4489 | |
| pubs.author-url | ||
| pubs.begin-page | 15116 | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Science & Society | |
| pubs.organisational-group | Initiatives | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Surgery | |
| pubs.organisational-group | Surgery, Plastic, Maxillofacial, and Oral Surgery | |
| pubs.publication-status | Published online | |
| pubs.volume | 5 |
Files
Original bundle
- Name:
- Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.pdf
- Size:
- 1.63 MB
- Format:
- Adobe Portable Document Format
- Description:
- Published version