Identification of fluorescent beads using a coded aperture snapshot spectral imager.

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2010-04-01

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Abstract

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSI's 2D bead identity map.

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Brady

David J. Brady

Michael J. Fitzpatrick Distinguished Professor Emeritus of Photonics

David Brady leads the Duke Information Spaces Project (DISP). Historically, DISP has focused on computational imaging systems, with particular emphasis on smart cameras for security, consumer, transportation and broadcast applications. Currently DISP focuses primarily on the use of artificial intelligence in camera arrays for interactive broadcasting.


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