Identification of fluorescent beads using a coded aperture snapshot spectral imager.

dc.contributor.author

Cull, Christy Fernandez

dc.contributor.author

Choi, Kerkil

dc.contributor.author

Brady, David J

dc.contributor.author

Oliver, Tim

dc.coverage.spatial

United States

dc.date.accessioned

2011-06-21T17:27:39Z

dc.date.issued

2010-04-01

dc.description.abstract

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSI's 2D bead identity map.

dc.description.version

Version of Record

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/20357842

dc.identifier

196222

dc.identifier.eissn

1539-4522

dc.identifier.uri

https://hdl.handle.net/10161/4204

dc.language

eng

dc.language.iso

en_US

dc.publisher

The Optical Society

dc.relation.ispartof

Appl Opt

dc.relation.journal

Applied Optics

dc.subject

Algorithms

dc.subject

Fluorescent Dyes

dc.subject

Imaging, Three-Dimensional

dc.subject

Microspheres

dc.subject

Optical Devices

dc.subject

Optical Phenomena

dc.subject

Spectrometry, Fluorescence

dc.title

Identification of fluorescent beads using a coded aperture snapshot spectral imager.

dc.title.alternative
dc.type

Journal article

duke.date.pubdate

2010-4-1

duke.description.issue

10

duke.description.volume

49

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/20357842

pubs.begin-page

B59

pubs.end-page

B70

pubs.issue

10

pubs.organisational-group

Duke

pubs.organisational-group

Electrical and Computer Engineering

pubs.organisational-group

Pratt School of Engineering

pubs.publication-status

Published

pubs.volume

49

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