Cyanobacterial ribosomal RNA genes with multiple, endonuclease-encoding group I introns.

Loading...
Thumbnail Image

Date

2007-09-08

Journal Title

Journal ISSN

Volume Title

Repository Usage Stats

60
views
14
downloads

Citation Stats

Abstract

Group I introns are one of the four major classes of introns as defined by their distinct splicing mechanisms. Because they catalyze their own removal from precursor transcripts, group I introns are referred to as autocatalytic introns. Group I introns are common in fungal and protist nuclear ribosomal RNA genes and in organellar genomes. In contrast, they are rare in all other organisms and genomes, including bacteria.Here we report five group I introns, each containing a LAGLIDADG homing endonuclease gene (HEG), in large subunit (LSU) rRNA genes of cyanobacteria. Three of the introns are located in the LSU gene of Synechococcus sp. C9, and the other two are in the LSU gene of Synechococcus lividus strain C1. Phylogenetic analyses show that these introns and their HEGs are closely related to introns and HEGs located at homologous insertion sites in organellar and bacterial rDNA genes. We also present a compilation of group I introns with homing endonuclease genes in bacteria.We have discovered multiple HEG-containing group I introns in a single bacterial gene. To our knowledge, these are the first cases of multiple group I introns in the same bacterial gene (multiple group I introns have been reported in at least one phage gene and one prophage gene). The HEGs each contain one copy of the LAGLIDADG motif and presumably function as homodimers. Phylogenetic analysis, in conjunction with their patchy taxonomic distribution, suggests that these intron-HEG elements have been transferred horizontally among organelles and bacteria. However, the mode of transfer and the nature of the biological connections among the intron-containing organisms are unknown.

Department

Description

Provenance

Citation

Published Version (Please cite this version)

10.1186/1471-2148-7-159

Publication Info

Haugen, P, D Bhattacharya, JD Palmer, S Turner, LA Lewis and KM Pryer (2007). Cyanobacterial ribosomal RNA genes with multiple, endonuclease-encoding group I introns. BMC evolutionary biology, 7(1). p. 159. 10.1186/1471-2148-7-159 Retrieved from https://hdl.handle.net/10161/21736.

This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.

Scholars@Duke

Pryer

Kathleen M. Pryer

Professor of Biology

Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.