Partial splenectomy but not total splenectomy preserves immunoglobulin M memory B cells in mice.
dc.contributor.author | Tracy, Elisabeth T | |
dc.contributor.author | Haas, Karen M | |
dc.contributor.author | Gentry, Tracy | |
dc.contributor.author | Danko, Melissa | |
dc.contributor.author | Roberts, Joseph L | |
dc.contributor.author | Kurtzberg, Joanne | |
dc.contributor.author | Rice, Henry E | |
dc.date.accessioned | 2022-03-23T19:32:34Z | |
dc.date.available | 2022-03-23T19:32:34Z | |
dc.date.issued | 2011-09 | |
dc.date.updated | 2022-03-23T19:32:33Z | |
dc.description.abstract | PurposeThe mechanism by which partial splenectomy preserves splenic immune function is unknown. Immunoglobulin (Ig) M memory B cells are critical for the immune response against encapsulated bacteria and are reduced in asplenic patients, although it is unknown whether partial splenectomy can preserve memory B cells. We hypothesized that IgM memory B cells (murine B-1a cells) would be preserved after partial splenectomy but not after total splenectomy in mice.MethodsWe performed total splenectomy (n = 17), partial splenectomy (n = 10), or sham laparotomy (n = 16) on C57BL/6J mice. Mice were killed on postoperative day 10 or 30, and peritoneal washings were analyzed by multiparameter flow cytometry for expression of murine B-1a cells (IgM(pos)IgD(dull)CD5(pos)B220(dull)).ResultsWe found that B-1a cells were significantly reduced after both total and partial splenectomies compared with sham laparotomy in the early postoperative period, although normal levels of B-1a cells returned by postoperative day 30 in mice undergoing partial splenectomy but not total splenectomy.ConclusionPartial splenectomy but not total splenectomy preserves the B-1a B-cell population in mice within 30 days after surgery. Maintenance of these critical B cells may contribute to the preservation of a splenic-dependent immune response after partial splenectomy. | |
dc.identifier | S0022-3468(11)00322-8 | |
dc.identifier.issn | 0022-3468 | |
dc.identifier.issn | 1531-5037 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Elsevier BV | |
dc.relation.ispartof | Journal of pediatric surgery | |
dc.relation.isversionof | 10.1016/j.jpedsurg.2011.04.020 | |
dc.subject | B-Lymphocytes | |
dc.subject | Animals | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Mice | |
dc.subject | Immunoglobulin M | |
dc.subject | Splenectomy | |
dc.subject | Immunologic Memory | |
dc.title | Partial splenectomy but not total splenectomy preserves immunoglobulin M memory B cells in mice. | |
dc.type | Journal article | |
duke.contributor.orcid | Kurtzberg, Joanne|0000-0002-3370-0703 | |
duke.contributor.orcid | Rice, Henry E|0000-0001-8033-6687 | |
pubs.begin-page | 1706 | |
pubs.end-page | 1710 | |
pubs.issue | 9 | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.organisational-group | Clinical Science Departments | |
pubs.organisational-group | Institutes and Centers | |
pubs.organisational-group | Pathology | |
pubs.organisational-group | Pediatrics | |
pubs.organisational-group | Surgery | |
pubs.organisational-group | Pediatrics, Allergy and Immunology | |
pubs.organisational-group | Surgery, Pediatric General Surgery | |
pubs.organisational-group | Duke Cancer Institute | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.organisational-group | University Institutes and Centers | |
pubs.organisational-group | Duke Global Health Institute | |
pubs.organisational-group | Initiatives | |
pubs.organisational-group | Duke Innovation & Entrepreneurship | |
pubs.organisational-group | Pediatrics, Transplant and Cellular Therapy | |
pubs.publication-status | Published | |
pubs.volume | 46 |
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