Partial splenectomy but not total splenectomy preserves immunoglobulin M memory B cells in mice.

dc.contributor.author

Tracy, Elisabeth T

dc.contributor.author

Haas, Karen M

dc.contributor.author

Gentry, Tracy

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Danko, Melissa

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Roberts, Joseph L

dc.contributor.author

Kurtzberg, Joanne

dc.contributor.author

Rice, Henry E

dc.date.accessioned

2022-03-23T19:32:34Z

dc.date.available

2022-03-23T19:32:34Z

dc.date.issued

2011-09

dc.date.updated

2022-03-23T19:32:33Z

dc.description.abstract

Purpose

The mechanism by which partial splenectomy preserves splenic immune function is unknown. Immunoglobulin (Ig) M memory B cells are critical for the immune response against encapsulated bacteria and are reduced in asplenic patients, although it is unknown whether partial splenectomy can preserve memory B cells. We hypothesized that IgM memory B cells (murine B-1a cells) would be preserved after partial splenectomy but not after total splenectomy in mice.

Methods

We performed total splenectomy (n = 17), partial splenectomy (n = 10), or sham laparotomy (n = 16) on C57BL/6J mice. Mice were killed on postoperative day 10 or 30, and peritoneal washings were analyzed by multiparameter flow cytometry for expression of murine B-1a cells (IgM(pos)IgD(dull)CD5(pos)B220(dull)).

Results

We found that B-1a cells were significantly reduced after both total and partial splenectomies compared with sham laparotomy in the early postoperative period, although normal levels of B-1a cells returned by postoperative day 30 in mice undergoing partial splenectomy but not total splenectomy.

Conclusion

Partial splenectomy but not total splenectomy preserves the B-1a B-cell population in mice within 30 days after surgery. Maintenance of these critical B cells may contribute to the preservation of a splenic-dependent immune response after partial splenectomy.
dc.identifier

S0022-3468(11)00322-8

dc.identifier.issn

0022-3468

dc.identifier.issn

1531-5037

dc.identifier.uri

https://hdl.handle.net/10161/24657

dc.language

eng

dc.publisher

Elsevier BV

dc.relation.ispartof

Journal of pediatric surgery

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10.1016/j.jpedsurg.2011.04.020

dc.subject

B-Lymphocytes

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Animals

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Mice, Inbred C57BL

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Mice

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Immunoglobulin M

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Splenectomy

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Immunologic Memory

dc.title

Partial splenectomy but not total splenectomy preserves immunoglobulin M memory B cells in mice.

dc.type

Journal article

duke.contributor.orcid

Kurtzberg, Joanne|0000-0002-3370-0703

duke.contributor.orcid

Rice, Henry E|0000-0001-8033-6687

pubs.begin-page

1706

pubs.end-page

1710

pubs.issue

9

pubs.organisational-group

Duke

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School of Medicine

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Clinical Science Departments

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Institutes and Centers

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Pathology

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Pediatrics

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Surgery

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Pediatrics, Allergy and Immunology

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Surgery, Pediatric General Surgery

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Duke Cancer Institute

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Institutes and Provost's Academic Units

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University Institutes and Centers

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Duke Global Health Institute

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Initiatives

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Duke Innovation & Entrepreneurship

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Pediatrics, Transplant and Cellular Therapy

pubs.publication-status

Published

pubs.volume

46

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