Exploratory polarization facilitates mating partner selection in <i>Saccharomyces cerevisiae</i>.
Repository Usage Stats
Yeast decode pheromone gradients to locate mating partners, providing a model for chemotropism. How yeast polarize toward a single partner in crowded environments is unclear. Initially, cells often polarize in unproductive directions, but then they relocate the polarity site until two partners' polarity sites align, whereupon the cells "commit" to each other by stabilizing polarity to promote fusion. Here we address the role of the early mobile polarity sites. We found that commitment by either partner failed if just one partner was defective in generating, orienting, or stabilizing its mobile polarity sites. Mobile polarity sites were enriched for pheromone receptors and G proteins, and we suggest that such sites engage in an exploratory search of the local pheromone landscape, stabilizing only when they detect elevated pheromone levels. Mobile polarity sites were also enriched for pheromone secretion factors, and simulations suggest that only focal secretion at polarity sites would produce high pheromone concentrations at the partner's polarity site, triggering commitment.
Published Version (Please cite this version)
Clark-Cotton, Manuella R, Nicholas T Henderson, Michael Pablo, Debraj Ghose, Timothy C Elston and Daniel J Lew (2021). Exploratory polarization facilitates mating partner selection in Saccharomyces cerevisiae. Molecular biology of the cell, 32(10). pp. 1048–1063. 10.1091/mbc.e21-02-0068 Retrieved from https://hdl.handle.net/10161/24506.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.