Characterizing the role of the immune microenvironment in multiple myeloma progression at a single-cell level.

dc.contributor.author

Schinke, Carolina

dc.contributor.author

Poos, Alexandra M

dc.contributor.author

Bauer, Michael

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John, Lukas

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Johnson, Sarah

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Deshpande, Shayu

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Carrillo, Luis

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Alapat, Daisy

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Rasche, Leo

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Thanendrarajan, Sharmilan

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Zangari, Maurizio

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Al Hadidi, Samer

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van Rhee, Frits

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Davies, Faith

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Raab, Marc S

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Morgan, Gareth

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Weinhold, Niels

dc.date.accessioned

2024-02-27T02:41:53Z

dc.date.available

2024-02-27T02:41:53Z

dc.date.issued

2022-11

dc.description.abstract

Early alterations within the bone marrow microenvironment that contribute to the progression of multiple myeloma (MM) from its precursor stages could be the key to identifying novel therapeutic approaches. However, the intrinsic variability in cellular populations between patients and the differences in sample processing and analysis methods have made it difficult to identify consistent changes between data sets. Here, we used single-cell RNA sequencing of bone marrow cells from precursor stages, monoclonal gammopathy of unknown significance, smoldering MM, and newly diagnosed MM and analyzed our data in combination with a previously published data set that used a similar patient population and sample processing. Despite the vast interpatient heterogeneity, some alterations were consistently observed in both data sets. We identified changes in immune cell populations as the disease progressed, which were characterized by a substantial decrease in memory and naïve CD4 T cells, and an increase in CD8+ effector T cells and T-regulatory cells. These alterations were further accompanied by an enrichment of nonclonal memory B cells and an increase in CD14 and CD16 monocytes in MM compared with its precursor stages. These results provide crucial information on the immune changes associated with the progression to clinical MM and can help to develop immune-based strategies for patient stratification and early therapeutic intervention.

dc.identifier

486276

dc.identifier.issn

2473-9529

dc.identifier.issn

2473-9537

dc.identifier.uri

https://hdl.handle.net/10161/30197

dc.language

eng

dc.relation.ispartof

Blood advances

dc.relation.isversionof

10.1182/bloodadvances.2022007217

dc.rights.uri

https://creativecommons.org/licenses/by-nc/4.0

dc.subject

Bone Marrow

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Humans

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Multiple Myeloma

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Paraproteinemias

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Monoclonal Gammopathy of Undetermined Significance

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Tumor Microenvironment

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Smoldering Multiple Myeloma

dc.title

Characterizing the role of the immune microenvironment in multiple myeloma progression at a single-cell level.

dc.type

Journal article

duke.contributor.orcid

Carrillo, Luis|0000-0003-1045-7971

pubs.begin-page

5873

pubs.end-page

5883

pubs.issue

22

pubs.organisational-group

Duke

pubs.organisational-group

School of Medicine

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Pathology

pubs.publication-status

Published

pubs.volume

6

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