Characterization of topographical effects on macrophage behavior in a foreign body response model.

Loading...
Thumbnail Image

Date

2010-05

Journal Title

Journal ISSN

Volume Title

Repository Usage Stats

235
views
952
downloads

Citation Stats

Abstract

Current strategies to limit macrophage adhesion, fusion and fibrous capsule formation in the foreign body response have focused on modulating material surface properties. We hypothesize that topography close to biological scale, in the micron and nanometric range, provides a passive approach without bioactive agents to modulate macrophage behavior. In our study, topography-induced changes in macrophage behavior was examined using parallel gratings (250 nm-2 mum line width) imprinted on poly(epsilon-caprolactone) (PCL), poly(lactic acid) (PLA) and poly(dimethyl siloxane) (PDMS). RAW 264.7 cell adhesion and elongation occurred maximally on 500 nm gratings compared to planar controls over 48 h. TNF-alpha and VEGF secretion levels by RAW 264.7 cells showed greatest sensitivity to topographical effects, with reduced levels observed on larger grating sizes at 48 h. In vivo studies at 21 days showed reduced macrophage adhesion density and degree of high cell fusion on 2 mum gratings compared to planar controls. It was concluded that topography affects macrophage behavior in the foreign body response on all polymer surfaces examined. Topography-induced changes, independent of surface chemistry, did not reveal distinctive patterns but do affect cell morphology and cytokine secretion in vitro, and cell adhesion in vivo particularly on larger size topography compared to planar controls.

Department

Description

Provenance

Citation

Published Version (Please cite this version)

10.1016/j.biomaterials.2010.01.074

Publication Info

Chen, Sulin, Jacqueline A Jones, Yongan Xu, Hong-Yee Low, James M Anderson and Kam W Leong (2010). Characterization of topographical effects on macrophage behavior in a foreign body response model. Biomaterials, 31(13). pp. 3479–3491. 10.1016/j.biomaterials.2010.01.074 Retrieved from https://hdl.handle.net/10161/6932.

This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.


Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.