Gene expression disruptions of organism versus organ in Drosophila species hybrids.

dc.contributor.author

Catron, Daniel J

dc.contributor.author

Noor, Mohamed AF

dc.coverage.spatial

United States

dc.date.accessioned

2011-06-21T17:31:24Z

dc.date.issued

2008-08-20

dc.description.abstract

Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes.

dc.description.version

Version of Record

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/18714377

dc.identifier.eissn

1932-6203

dc.identifier.uri

https://hdl.handle.net/10161/4502

dc.language

eng

dc.language.iso

en_US

dc.publisher

Public Library of Science (PLoS)

dc.relation.ispartof

PLoS One

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10.1371/journal.pone.0003009

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Plos One

dc.subject

Animals

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Cyclin B

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Drosophila

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Drosophila Proteins

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Gene Expression Profiling

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Gene Expression Regulation

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Male

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Nucleic Acid Hybridization

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RNA, Messenger

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Spermatids

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Spermatogenesis

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Transcription, Genetic

dc.title

Gene expression disruptions of organism versus organ in Drosophila species hybrids.

dc.title.alternative
dc.type

Journal article

duke.date.pubdate

2008-8-20

duke.description.issue

8

duke.description.volume

3

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/18714377

pubs.begin-page

e3009

pubs.issue

8

pubs.organisational-group

Biology

pubs.organisational-group

Duke

pubs.organisational-group

Trinity College of Arts & Sciences

pubs.publication-status

Published online

pubs.volume

3

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