Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal.

Abstract

Background

Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. Resistance to antifungal agents has increased during the last decade. Thus, identification of Candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to speciate Candida species and to determine antifungal susceptibility pattern of Candida species to antifungal agents.

Methods

A total of 100 consecutive Candida species were isolated from 1248 clinical specimens over 7 months period. Growths on Sabouraud dextrose agar were evaluated for colony appearance, macroscopic examination, Gram staining, germ tube test and urea hydrolysis test. Further, they were processed for Candida speciation on CHROMagar. Antifungal susceptibility testing was performed as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document.

Results

Out of 100 Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans Candida species, Candida tropicalis (20%) was the predominant isolate followed by Candida glabrata (14%). Regarding antifungal susceptibility pattern, Candida species were more susceptible to clotrimazole (82%) followed by fluconazole (64%) and miconazole (44%).

Conclusions

Candida albicans was the predominant species responsible for various Candidal infections. Among commonly used antifungal drugs clotrimazole, miconazole and fluconazole were most effective.

Department

Description

Provenance

Citation

Published Version (Please cite this version)

10.1186/s13104-017-2547-3

Publication Info

Khadka, Sundar, Jeevan Bahadur Sherchand, Bharat Mani Pokhrel, Keshab Parajuli, Shyam Kumar Mishra, Sangita Sharma, Niranjan Shah, Hari Prasad Kattel, et al. (2017). Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal. BMC research notes, 10(1). p. 218. 10.1186/s13104-017-2547-3 Retrieved from https://hdl.handle.net/10161/27409.

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Scholars@Duke

Khadka

Sundar Khadka

Postdoctoral Associate

Postdoctoral Research Associate, Shinohara laboratory


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