In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast.
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2005-07
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Abstract
Clathrin-mediated transport is a major pathway for endocytosis. However, in yeast, where cortical actin patches are essential for endocytosis, plasma membrane-associated clathrin has never been observed. Using live cell imaging, we demonstrate cortical clathrin in association with the actin-based endocytic machinery in yeast. Fluorescently tagged clathrin is found in highly mobile internal trans-Golgi/endosomal structures and in smaller cortical patches. Total internal reflection fluorescence microscopy showed that cortical patches are likely endocytic sites, as clathrin is recruited prior to a burst of intensity of the actin patch/endocytic marker, Abp1. Clathrin also accumulates at the cortex with internalizing alpha factor receptor, Ste2p. Cortical clathrin localizes with epsins Ent1/2p and AP180s, and its recruitment to the surface is dependent upon these adaptors. In contrast, Sla2p, End3p, Pan1p, and a dynamic actin cytoskeleton are not required for clathrin assembly or exchange but are required for the mobility, maturation, and/or turnover of clathrin-containing endocytic structures.
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Newpher, Thomas M, Robin P Smith, Vance Lemmon and Sandra K Lemmon (2005). In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast. Developmental cell, 9(1). pp. 87–98. 10.1016/j.devcel.2005.04.014 Retrieved from https://hdl.handle.net/10161/27392.
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Thomas Mark Newpher
I teach, mentor, and advise in Duke’s Neuroscience major, and serve as the Associate Director of Undergraduate Studies in Neuroscience. I also direct the Summer Neuroscience Program of Research, where I provide mentorship and professional development opportunities for undergraduate research fellows. I earned my B.A. in Biology from Thiel College and my Ph.D. in Molecular Biology and Microbiology from Case Western Reserve University. In addition, I received postdoctoral training in the Departments of Neurobiology and Cell Biology at Duke University, where my research focused on the molecular mechanisms that underlie learning-related synaptic plasticity.
As a faculty member in the Department of Psychology and Neuroscience I teach several courses, including Cellular and Molecular Neurobiology (NEUROSCI 223), Contemporary Neuroscience Methods (NEUROSCI 376), the Neurobiology of Learning and Memory (NEUROSCI 461S), and Neuroplasticity and Disease (NEUROSCI 353S). My courses use a variety of team-based learning activities to promote critical thinking skills, foster collaboration among students, and create an engaging, student-centered classroom experience. As a co-PI in the Duke Team-Based Learning lab, I study the impacts of collaborative learning on student performance and classroom dynamics.
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