Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring

Vidal, AC

Murphy, SK

Murtha, AP

Schildkraut, JM

Soubry, A

Huang, Z

Neelon, SEB

Fuemmeler, B

Iversen, E

Wang, F

Kurtzberg, J

Jirtle, RL

Hoyo, C

2022-03-23T19:40:52Z

2022-03-23T19:40:52Z

2013

2022-03-23T19:40:51Z

Objectives: Low birth weight (LBW) has been associated with common adult-onset chronic diseases, including obesity, cardiovascular disease, type II diabetes and some cancers. The etiology of LBW is multi-factorial. However, recent evidence suggests exposure to antibiotics may also increase the risk of LBW. The mechanisms underlying this association are unknown, although epigenetic mechanisms are hypothesized. In this study, we evaluated the association between maternal antibiotic use and LBW and examined the potential role of altered DNA methylation that controls growth regulatory imprinted genes in these associations. Methods: Between 2009-2011, 397 pregnant women were enrolled and followed until delivery. Prenatal antibiotic use was ascertained through maternal self-report. Imprinted genes methylation levels were measured at differentially methylated regions (DMRs) using bisulfite pyrosequencing. Generalized linear models were used to examine associations among antibiotic use, birth weight and DMR methylation fractions. Results: After adjusting for infant gender, race/ethnicity, maternal body mass index, delivery route, gestational weight gain, gestational age at delivery, folic acid intake, physical activity, maternal smoking and parity, antibiotic use during pregnancy was associated with 138 g lower birth weight compared with non-antibiotic use (β-coefficient=-132.99, s.e.=50.70, P=0.008). These associations were strongest in newborns of women who reported antibiotic use other than penicillins (β-coefficient=-135.57, s.e.=57.38, P=0.02). Methylation at five DMRs, IGF2 (P=0.05), H19 (P=0.15), PLAGL1 (P=0.01), MEG3 (P=0.006) and PEG3 (P=0.08), was associated with maternal antibiotic use; among these, only methylation at the PLAGL1 DMR was also associated with birth weight. Conclusion: We report an inverse association between in utero exposure to antibiotics and lower infant birth weight and provide the first empirical evidence supporting imprinted gene plasticity in these associations. © 2013 Macmillan Publishers Limited.

0307-0565

https://hdl.handle.net/10161/24664

International Journal of Obesity

10.1038/ijo.2013.47

Associations between antibiotic exposure during pregnancy, birth weight and aberrant methylation at imprinted genes among offspring

Journal article

Murphy, SK|0000-0001-8298-7272

Kurtzberg, J|0000-0002-3370-0703

907

913

7

Duke

Nicholas School of the Environment

Sanford School of Public Policy

School of Medicine

Trinity College of Arts & Sciences

Faculty

Clinical Science Departments

Institutes and Centers

Family Medicine and Community Health

Pathology

Pediatrics

Family Medicine and Community Health, Prevention Research

Pediatrics, Primary Care Pediatrics

Duke Cancer Institute

Statistical Science

Environmental Sciences and Policy

Institutes and Provost's Academic Units

Initiatives

Center for Child and Family Policy

Duke Innovation & Entrepreneurship

Pediatrics, Transplant and Cellular Therapy

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