Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.

dc.contributor.author

Meadows, Sarah K

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Dressman, Holly K

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Daher, Pamela

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Himburg, Heather

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Russell, J Lauren

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Doan, Phuong

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Chao, Nelson J

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Lucas, Joseph

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Nevins, Joseph R

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Chute, John P

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United States

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2011-06-21T17:31:32Z

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2010-07-12

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In the event of a terrorist-mediated attack in the United States using radiological or improvised nuclear weapons, it is expected that hundreds of thousands of people could be exposed to life-threatening levels of ionizing radiation. We have recently shown that genome-wide expression analysis of the peripheral blood (PB) can generate gene expression profiles that can predict radiation exposure and distinguish the dose level of exposure following total body irradiation (TBI). However, in the event a radiation-mass casualty scenario, many victims will have heterogeneous exposure due to partial shielding and it is unknown whether PB gene expression profiles would be useful in predicting the status of partially irradiated individuals. Here, we identified gene expression profiles in the PB that were characteristic of anterior hemibody-, posterior hemibody- and single limb-irradiation at 0.5 Gy, 2 Gy and 10 Gy in C57Bl6 mice. These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79-100%) compared to non-irradiated mice. Interestingly, PB signatures of partial body irradiation were poorly predictive of radiation status by site of injury (range 16-43%), suggesting that the PB molecular response to partial body irradiation was anatomic site specific. Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls. These data demonstrate that partial body irradiation, even to a single limb, generates a characteristic PB signature of radiation injury and thus may necessitate the use of multiple signatures, both partial body and total body, to accurately assess the status of an individual exposed to radiation.

dc.description.version

Version of Record

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http://www.ncbi.nlm.nih.gov/pubmed/20634956

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1932-6203

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https://hdl.handle.net/10161/4550

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eng

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en_US

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Public Library of Science (PLoS)

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PLoS One

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10.1371/journal.pone.0011535

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Plos One

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Animals

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Female

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Gene Expression

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Gene Expression Profiling

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Leukocytes, Mononuclear

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Mice

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Mice, Inbred C57BL

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Oligonucleotide Array Sequence Analysis

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Radiation Injuries, Experimental

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Radiation, Ionizing

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Whole-Body Irradiation

dc.title

Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.

dc.title.alternative
dc.type

Journal article

duke.contributor.orcid

Doan, Phuong|0000-0003-1361-2068

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Chao, Nelson J|0000-0001-6725-7220

duke.date.pubdate

2010-7-12

duke.description.issue

7

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5

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/20634956

pubs.begin-page

e11535

pubs.issue

7

pubs.organisational-group

Basic Science Departments

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Biostatistics & Bioinformatics

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Clinical Science Departments

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Duke

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Duke Cancer Institute

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Faculty

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Global Health Institute

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Immunology

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Institutes and Centers

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Institutes and Provost's Academic Units

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Medicine

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Medicine, Cellular Therapy

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Medicine, Hematological Malignancies

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Molecular Genetics and Microbiology

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Pathology

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School of Medicine

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Social Science Research Institute

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University Institutes and Centers

pubs.publication-status

Published online

pubs.volume

5

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