An enhanced isothermal amplification assay for viral detection.
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2020-11
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Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability.
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Qian, Jason, Sarah A Boswell, Christopher Chidley, Zhi-Xiang Lu, Mary E Pettit, Benjamin L Gaudio, Jesse M Fajnzylber, Ryan T Ingram, et al. (2020). An enhanced isothermal amplification assay for viral detection. Nature communications, 11(1). p. 5920. 10.1038/s41467-020-19258-y Retrieved from https://hdl.handle.net/10161/31263.
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Christopher Chidley
Chris Chidley is an incoming Assistant Professor in the Department of Pharmacology and Cancer Biology starting in September 2024.
The Chidley lab investigates the role of transporter proteins in nutrient and metabolite import/export and their impact on cell state and response to chemotherapeutics. The lab uses unbiased genetic screening in cell and animal models along with biochemistry, cell biology, and analytical chemistry tools to identify critical small molecule transporters across different environments, characterize their function, and explore how the tumor microenvironment impacts cancer metabolism. Our research aims to guide the development of metabolic strategies to suppress tumor growth, and aid precision medicine efforts by revealing determinants of drug sensitivity.
For more information, visit our lab website.
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